Separation of Line Widths of HOD Peaks of Healthy and Diseased Blood and Urine Groups Using 400 MHz NMR

Abstract

Line width studies of high field nuclear magnetic resonance (NMR) peaks have been seen more frequently in the recent literature. This study aims to compare the NMR line widths of HOD peaks of healthy and diseased blood, and densely bloody cysts taken from the jaw. Comparisons of HOD peak line widths in healthy and diseased urine were also made. For this purpose, the blood, serum, and urine of 29 cancer patients, 17 diabetic patients, 28 healthy volunteers, and 20 intensely bloody samples from jaw cysts were collected. Mixtures were prepared by adding the 0.02-mL sample to 0.98 mL D₂O. Single-pulse proton NMR measurements were performed at 400 MHz, and line widths were obtained from the half-height of the blood HOD peak. Statistical evaluations show that the line width of cancerous blood is different from normal blood (P = 0.032), while the line width of diabetic blood is not different (p = 0.072), whereas other groups and bloody jaw cysts are completely different (p < 0.05). Similar results were found for urine groups. Line widths show a moderate correlation with each of the albumin and total protein groups (R around 0,56). Present data suggest that a comparison of healthy and diseased body fluids can be made by line width measurements of the HOD peak in the high NMR field. Data also suggest that the relaxivities of albumin (Ser-Alb), total protein (Ser-TP) are potential diagnostic indicators for cancerous blood.