Potential of Industrial Waste to Transfer Microbial Electron in Microbial Fuel Cell Using Dye Reduction Assay

IF 3 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES Water, Air, & Soil Pollution Pub Date : 2025-01-23 DOI:10.1007/s11270-025-07765-x

Ann Maxton, Sam A. Masih

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Abstract

Industries are pillar for nation development, however their development comes with environmental disturbance. Industrial discharge contributes majorly in water pollution however this microbial culture, organic matter rich water serves as an excellent anolyte for Microbial Fuel Cell (MFC). Transfer of electrons is a crucial step in bio electrochemical process catalyzed by microbial community. Methylene Blue reduction method could be an game changer in MFC operations using dye reduction-based electron-transfer activity monitoring (DREAM) assay. Several industrial waste water samples were tested and noticed textile waste water as best suited for DREAM coefficient and electron transportation among other two selected (paper and paint). On screening various microbial dilutions (1x—0.125x) for optimization of DREAM coefficient for maximum power output, the necessity of active microbial populations along with metabolic state was confirmed. Additionally, on screening carbon source suitability (acetate, glucose and sucrose), it was established that maximum DREAM coefficient (0.66 ± 0.03) and power density (91.92 ± 0.81 mW/m²) was achieved with acetate as it is end product of numerous metabolic pathways. Optimized concentration of the best suited carbon source (i.e. acetate) was also analysed and recorded as 25 mmol/L generating maximum power output during MFC operation. Our results established and confirmed that novel DREAM assay as an appropriate, cost effective approach to estimate overall microbial electron exchange as it directly correlates with viable cell count and microbial activity of industrial waste water sample in MFC operation to analyze electrogenic microbial population responsible for bioremediation and green energy production.

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利用染料还原法研究工业废物在微生物燃料电池中转移微生物电子的潜力

工业是国家发展的支柱，但工业的发展也伴随着环境的干扰。工业排放是造成水污染的主要原因，而这种富含有机物的微生物培养水是微生物燃料电池（MFC）的优良阳极液。在微生物催化的生物电化学过程中，电子转移是一个至关重要的环节。使用基于染料还原的电子转移活性监测（DREAM）分析，亚甲基蓝还原方法可能会改变MFC操作的游戏规则。对几个工业废水样品进行了测试，并注意到纺织废水是最适合DREAM系数和电子传输的其他两种选择（纸张和油漆）。通过筛选不同的微生物稀释度（1x-0.125x）来优化DREAM系数以获得最大功率输出，确认了活性微生物种群与代谢状态的必要性。此外，在筛选碳源适宜性（乙酸、葡萄糖和蔗糖）时，发现乙酸作为多种代谢途径的最终产物，DREAM系数（0.66±0.03）和功率密度（91.92±0.81 mW/m2）最大。还分析了最适合的碳源（即醋酸酯）的优化浓度，并记录为25 mmol/L，在MFC运行期间产生最大功率输出。我们的研究结果建立并证实了新的DREAM测定法是一种合适的、成本有效的方法，可以估计总体微生物电子交换，因为它与MFC操作中工业废水样品的活细胞计数和微生物活性直接相关，可以分析负责生物修复和绿色能源生产的电致微生物种群。

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来源期刊

Water, Air, & Soil Pollution 环境科学-环境科学

CiteScore

4.50

自引率

6.90%

发文量

448

审稿时长

2.6 months

期刊介绍： Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments. Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation. Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.