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Abstract

We engineered a specialized actinomycete cell factory, 306A, optimized for the efficient production of PoTeMs. By introducing the heterologous biosynthetic gene cluster fla from marine-derived Streptomyces flavogriseus SCSIO 40032—akin to a factory receiving a production order—the biosynthetic machinery functions like an assembly line. Flavin oxidase and P450 enzymes function as key "machines" on this line, catalyzing the cyclization of PoTeMs precursors, hydroxylation, and the formation of a C14 ketone group, ultimately yielding mature PoTeMs such as 10-epi-xanthobaccin C. More details are discussed in the article by Zhang et al. on pages 483—490.