Genetic Characterization of the Immortalized Human Nasopharyngeal Carcinoma Cell Line NPC/HK1

IF 3.1 2区医学 Q2 ONCOLOGY Cancer Medicine Pub Date : 2025-02-04 DOI:10.1002/cam4.70422

Anna Makowska, Udo Kontny, Josef van Helden, Barbara Hildebrandt, Herdit M. Schüler, Ralf Weiskirchen

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Abstract

Background

Human nasopharyngeal carcinoma (NPC) cell lines are in vitro model systems that are widely available, easy to handle, and provide an unlimited supply of material. They also bypass ethical concerns associated with the use of primary human cells or tissue. However, many of these cell lines including 5-8F, 6-10B, CNE-1, CNE-2, HNE-1, HONE-1, SUNE1, SUNE2, and NPC-TW01 have been shown to be misidentified or cross-contaminated. While simple molecular genotyping techniques such as short tandem repeat profiling of human cell lines are available to confirm cell line identity, scientists often do not implement strategies to avoid misidentification. This has resulted in a large volume of publications containing incorrect information.

Methods

In this paper, we have established a cell line karyogram that contains several marker chromosomes and a set of typical aberrations characteristic of NPC/HK1.

Results and Conclusions

Combined with the typical multiloci short tandem repeat signature of NPC/HK1, the cytogenetic analysis provides an effective means to avoid unreliable experimental outcomes and scientific misinterpretation.

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人鼻咽癌永生化细胞系NPC/HK1的遗传特性研究

人鼻咽癌（NPC）细胞系是一种体外模型系统，广泛可用，易于处理，并提供无限的材料供应。它们还绕过了与使用原代人类细胞或组织有关的伦理问题。然而，包括5-8F、6-10B、CNE-1、CNE-2、HNE-1、HONE-1、SUNE1、SUNE2和NPC-TW01在内的许多细胞系已被证明被错误识别或交叉污染。虽然人类细胞系的短串联重复谱等简单的分子基因分型技术可用于确认细胞系的身份，但科学家通常没有实施避免错误识别的策略。这导致大量出版物含有不正确的信息。方法建立了一种含有几条标记染色体和一组典型NPC/HK1染色体畸变的细胞系核图。结果与结论结合NPC/HK1典型的多位点短串联重复特征，细胞遗传学分析为避免实验结果不可靠和科学误读提供了有效手段。

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来源期刊

Cancer Medicine ONCOLOGY-

CiteScore

5.50

自引率

2.50%

发文量

907

审稿时长

19 weeks

期刊介绍： Cancer Medicine is a peer-reviewed, open access, interdisciplinary journal providing rapid publication of research from global biomedical researchers across the cancer sciences. The journal will consider submissions from all oncologic specialties, including, but not limited to, the following areas: Clinical Cancer Research Translational research ∙ clinical trials ∙ chemotherapy ∙ radiation therapy ∙ surgical therapy ∙ clinical observations ∙ clinical guidelines ∙ genetic consultation ∙ ethical considerations Cancer Biology: Molecular biology ∙ cellular biology ∙ molecular genetics ∙ genomics ∙ immunology ∙ epigenetics ∙ metabolic studies ∙ proteomics ∙ cytopathology ∙ carcinogenesis ∙ drug discovery and delivery. Cancer Prevention: Behavioral science ∙ psychosocial studies ∙ screening ∙ nutrition ∙ epidemiology and prevention ∙ community outreach. Bioinformatics: Gene expressions profiles ∙ gene regulation networks ∙ genome bioinformatics ∙ pathwayanalysis ∙ prognostic biomarkers. Cancer Medicine publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper.