Comparison of two commercial media for corneal organ culture and deswelling: CorneaMax/CorneaJet versus Tissue-C/Carry C

IF 3 3区医学 Q1 OPHTHALMOLOGY Acta Ophthalmologica Pub Date : 2025-01-19 DOI:10.1111/aos.17251

Zhiguo He, Sandrine Ninotta, David Toubeau, Tomy Sagnial, Sylvain Poinard, Oliver Dorado Cortez, Paul Goin, Philippe Gain, Marc Muraine, Gilles Thuret

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Abstract

Aims/Purpose: Corneal storage in organ culture (OC) at 31-34°C is the reference method in Europe. In France, eye banks have traditionally used CorneaMax/CorneaJet media (Eurobio, Les Ulis, France) for several decades. Recently, Tissue-C/Carry-C media (Alchimia-Moria, Antony, France) became available. This study aims at comparing the quality of corneas stored in these two media.

Methods: Eye banks in Saint Etienne and Rouen participated in the study. In Rouen, two experiments were conducted. The first included 18 pairs of corneas, with endothelial cell density (ECD) measured by routine eye bank methods on days -10 and -30 of OC, and 4 days after deswelling. In the second experiment, the protocol for Tissue-C was modified by renewing the medium on day-10. ECD was measured on days -10 and -22 of OC, and 4 days after deswelling. The ECD of 10 pairs of corneas was measured on days -4 and -28 at the Eye Bank of Saint-Etienne. After 2 days of deswelling, the viable ECD was assessed using Hoechst-Calcein-AM staining. Corneal thickness was also measured.

Results: In the first experiment in Rouen, ECD was higher in CorneaMax on day-30 (2202 ± 403 vs 2004 ± 434 cells/mm², p = 0.008) compared to Tissue-C, and higher in CorneaJet after 4 days of deswelling (2088 ± 399 vs 1852 ± 419 cells/mm², p = 0.019) compared to Carry-C. In the second experiment in Rouen, with the medium renewal for Tissue-C and reduced OC duration, the ECD difference disappeared. In Saint Etienne, with medium renewal for Tissue-C during OC, no significant difference in ECD was found during OC. However, the viable ECD of corneas deswelled in CorneaJet was significantly higher than in Carry-C (1647 ± 324 vs 1436 ± 235 cells/mm², p = 0.018).

Conclusions: CorneaMax recommends renewing the medium after the first ECD count, while Tissue-C does not. When following these instructions, CorneaMax showed better ECD results than Tissue-C. However, when the medium for Tissue-C was also renewed during OC, the ECD difference during OC disappeared. The viable ECD indicated that CorneaJet, a deswelling medium was superior to Carry-C. This could be related to the greater thickness of corneas in Tissue-C during OC.

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来源期刊

Acta Ophthalmologica 医学-眼科学

CiteScore

7.60

自引率

5.90%

发文量

433

审稿时长

6 months

期刊介绍： Acta Ophthalmologica is published on behalf of the Acta Ophthalmologica Scandinavica Foundation and is the official scientific publication of the following societies: The Danish Ophthalmological Society, The Finnish Ophthalmological Society, The Icelandic Ophthalmological Society, The Norwegian Ophthalmological Society and The Swedish Ophthalmological Society, and also the European Association for Vision and Eye Research (EVER). Acta Ophthalmologica publishes clinical and experimental original articles, reviews, editorials, educational photo essays (Diagnosis and Therapy in Ophthalmology), case reports and case series, letters to the editor and doctoral theses.