Citicoline and coenzyme Q10 (COQUN Combo) reduce inflammation in an experimental glaucoma model

IF 3 3区医学 Q1 OPHTHALMOLOGY Acta Ophthalmologica Pub Date : 2025-01-19 DOI:10.1111/aos.17112

José A. Matamoros, José A. Fernández-Albarral, Elena Salobrar-Garcia, Lorena Elvira-Hurtado, Inés López-Cuenca, Lidia Sanchez-Puebla, Miguel A. Martínez-López, Sara Rubio-Casado, Rosa de Hoz, Meritxell Lopez Gallardo, Eva M. Marco, Jose Manuel Ramirez, Juan Jose Salazar, Pedro De La Villa, Ana Isabel Ramirez

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引用次数: 0

Abstract

Aims/Purpose: To investigate the anti-inflammatory effects of citicoline and coenzyme Q10 (COQUN Combo) in an experimental model of ocular hypertension (OHT).

Methods: Swiss albino mice were used: vehicle control (Vehicle n = 6), COQUN Combo control (COQUN Combo n = 5), laser vehicle (LG+Veh, n = 6), and laser COQUN Combo (LG+COQUN Combo, n = 6). COQUN Combo was administered daily with gelatin 15 days prior to laser induction and 3 days post-induction in COQUN Combo and LG+COQUN Combo groups. The Vehicle and LG+Veh groups received neutral gelatin. OHT was induced in the left eye by laser photocoagulation of the limbal and episcleral veins, analyzing hypertensive eyes and their contralateral eyes. Retinal whole-mounts were processed with: 1) Anti Iba-1 to quantify: Number of Iba-1+ cells (Iba1-cn) in outer segments (OS), outer plexiform layer (OPL) and inner plexiform layer (IPL); Retinal area occupied by Iba-1+ cells (Iba1-RA) in the nerve fiber layer-ganglion cell layer (NFL-GCL); Cell body area (Iba1-cba) of Iba-1+ cells in OPL, IPL and NFL-GCL; and arbor area (Iba1-aa) of Iba-1+ cells in OPL and IPL. 2) Anti-P2RY12 to differentiate activated microglia.

Results: No significant differences were observed between the control groups. Both OHT eyes and their contralaterals of LG+Veh, as well as LG+COQUN Combo OHT eyes and their contralaterals, showed significant differences compared to controls in Iba1-cn, Iba1- RA, Iba1-cba, and Iba1-aa in all layers analyzed. However, these changes were significantly smaller in LG+COQUN Combo OHT and their contralateral eyes compared to LG+Veh OHT and their contralateral eyes. In the Vehicle, Iba-1+ cells were P2RY12+. P2RY12 expression was downregulated in both OHT eyes of LG+Veh and LG+ COQUN Combo; however, this downregulation was less pronounced in the OHT eyes of LG+COQUN Combo. In the contralateral eyes, P2RY12 expression remained similar to that of the controls

Conclusions: COQUN Combo can attenuate the inflammatory process in glaucoma.

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来源期刊

Acta Ophthalmologica 医学-眼科学

CiteScore

7.60

自引率

5.90%

发文量

433

审稿时长

6 months

期刊介绍： Acta Ophthalmologica is published on behalf of the Acta Ophthalmologica Scandinavica Foundation and is the official scientific publication of the following societies: The Danish Ophthalmological Society, The Finnish Ophthalmological Society, The Icelandic Ophthalmological Society, The Norwegian Ophthalmological Society and The Swedish Ophthalmological Society, and also the European Association for Vision and Eye Research (EVER). Acta Ophthalmologica publishes clinical and experimental original articles, reviews, editorials, educational photo essays (Diagnosis and Therapy in Ophthalmology), case reports and case series, letters to the editor and doctoral theses.