Citicoline and coenzyme Q10 (COQUN Combo) reduce inflammation in an experimental glaucoma model

IF 2.8 3区医学 Q1 OPHTHALMOLOGY Acta Ophthalmologica Pub Date : 2025-01-19 DOI:10.1111/aos.17112

José A. Matamoros, José A. Fernández-Albarral, Elena Salobrar-Garcia, Lorena Elvira-Hurtado, Inés López-Cuenca, Lidia Sanchez-Puebla, Miguel A. Martínez-López, Sara Rubio-Casado, Rosa de Hoz, Meritxell Lopez Gallardo, Eva M. Marco, Jose Manuel Ramirez, Juan Jose Salazar, Pedro De La Villa, Ana Isabel Ramirez

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Abstract

Aims/Purpose: To investigate the anti-inflammatory effects of citicoline and coenzyme Q10 (COQUN Combo) in an experimental model of ocular hypertension (OHT).

Methods: Swiss albino mice were used: vehicle control (Vehicle n = 6), COQUN Combo control (COQUN Combo n = 5), laser vehicle (LG+Veh, n = 6), and laser COQUN Combo (LG+COQUN Combo, n = 6). COQUN Combo was administered daily with gelatin 15 days prior to laser induction and 3 days post-induction in COQUN Combo and LG+COQUN Combo groups. The Vehicle and LG+Veh groups received neutral gelatin. OHT was induced in the left eye by laser photocoagulation of the limbal and episcleral veins, analyzing hypertensive eyes and their contralateral eyes. Retinal whole-mounts were processed with: 1) Anti Iba-1 to quantify: Number of Iba-1+ cells (Iba1-cn) in outer segments (OS), outer plexiform layer (OPL) and inner plexiform layer (IPL); Retinal area occupied by Iba-1+ cells (Iba1-RA) in the nerve fiber layer-ganglion cell layer (NFL-GCL); Cell body area (Iba1-cba) of Iba-1+ cells in OPL, IPL and NFL-GCL; and arbor area (Iba1-aa) of Iba-1+ cells in OPL and IPL. 2) Anti-P2RY12 to differentiate activated microglia.

Results: No significant differences were observed between the control groups. Both OHT eyes and their contralaterals of LG+Veh, as well as LG+COQUN Combo OHT eyes and their contralaterals, showed significant differences compared to controls in Iba1-cn, Iba1- RA, Iba1-cba, and Iba1-aa in all layers analyzed. However, these changes were significantly smaller in LG+COQUN Combo OHT and their contralateral eyes compared to LG+Veh OHT and their contralateral eyes. In the Vehicle, Iba-1+ cells were P2RY12+. P2RY12 expression was downregulated in both OHT eyes of LG+Veh and LG+ COQUN Combo; however, this downregulation was less pronounced in the OHT eyes of LG+COQUN Combo. In the contralateral eyes, P2RY12 expression remained similar to that of the controls

Conclusions: COQUN Combo can attenuate the inflammatory process in glaucoma.

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胞胆碱和辅酶Q10 （COQUN组合）减少实验性青光眼模型的炎症

目的：探讨胞胆碱和辅酶Q10 （COQUN Combo）对高眼压（OHT）实验模型的抗炎作用。方法：采用瑞士白化小鼠：小鼠对照（vehicle n = 6）、COQUN Combo对照（COQUN Combo n = 5）、激光对照（LG+Veh, n = 6）、激光COQUN Combo （LG+COQUN Combo, n = 6）。COQUN Combo组和LG+COQUN Combo组在激光诱导前15天和诱导后3天每天用明胶给予COQUN Combo。Vehicle组和LG+Veh组给予中性明胶。采用激光凝固左眼角膜缘静脉和巩膜外静脉诱导OHT，分析高血压眼及其对侧眼。用Anti - Iba-1处理视网膜全载：1)定量测定：外节段（OS）、外丛状层（OPL）和内丛状层（IPL） Iba-1+细胞（Iba1-cn）数量；神经纤维层-神经节细胞层（NFL-GCL）中Iba-1+细胞（Iba1-RA）占据视网膜区域；OPL、IPL和NFL-GCL中Iba-1+细胞的胞体面积（Iba1-cba）；和Iba-1+细胞在OPL和IPL中的乔木面积（Iba1-aa）。2)抗p2ry12分化活化小胶质细胞。结果：两组间无明显差异。LG+Veh组OHT眼及其对侧、LG+COQUN Combo组OHT眼及其对侧在各层的Iba1-cn、Iba1- RA、Iba1-cba、Iba1-aa与对照组相比均有显著差异。然而，与LG+Veh OHT及其对侧眼睛相比，LG+COQUN Combo OHT及其对侧眼睛的这些变化明显较小。在载体中，Iba-1+细胞为P2RY12+。LG+Veh和LG+ COQUN Combo的OHT眼P2RY12表达均下调；然而，这种下调在LG+COQUN组合的OHT眼中不太明显。在对侧眼，P2RY12的表达与对照组相似。结论：COQUN复方可减轻青光眼的炎症过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Ophthalmologica 医学-眼科学

CiteScore

7.60

自引率

5.90%

发文量

433

审稿时长

6 months

期刊介绍： Acta Ophthalmologica is published on behalf of the Acta Ophthalmologica Scandinavica Foundation and is the official scientific publication of the following societies: The Danish Ophthalmological Society, The Finnish Ophthalmological Society, The Icelandic Ophthalmological Society, The Norwegian Ophthalmological Society and The Swedish Ophthalmological Society, and also the European Association for Vision and Eye Research (EVER). Acta Ophthalmologica publishes clinical and experimental original articles, reviews, editorials, educational photo essays (Diagnosis and Therapy in Ophthalmology), case reports and case series, letters to the editor and doctoral theses.