INVESTIGATION OF THE POSSIBLE RELATIONSHIP OF INSEMINATED SPERM PROPERTIES IN ASSISTED REPRODUCTION TECHNIQUES CYCLES WITH EMBRYO MORPHOKINETIC PARAMETERS AND ANEUPLOIDY

IF 3.5 2区医学 Q1 OBSTETRICS & GYNECOLOGY Reproductive biomedicine online Pub Date : 2024-11-01 Epub Date: 2024-12-04 DOI:10.1016/j.rbmo.2024.104533

DUYGU KÜTÜK , ŞİRİN KİNETLİ , İBRAHİM ORÇUN OLCAY , TÜLAY İREZ , BERKAY AKÇAY , NECATİ FINDIKLI

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Abstract

Objective

This study aims to investigate and evaluate the aneuploidy status and morphokinetic relationships of embryos according to the sperm source used.

Methods

In our study, morphokinetic evaluation of embryos formed by testicular and ejaculate sperm was performed. Morphokinetic and PGD results of 1157 blastocysts obtained from 155 patients who applied to Bahçeci IVF center between 2016 and 2020 and had PGD indication were evaluated retrospectively according to ejaculate and testicular sperm usage.

Results

In morphokinetic evaluations, it was understood that the development of testicular sperm was shorter than the development of ejaculate sperm (p<0.05). It was shown that female age is effective in the development of aneuploid embryos in patients using both ejaculate and testicular sperm in the evaluation before and after the age of 35 (p<0.05). PGD analysis showed that the time lapse process in normal and abnormal embryos was longer in normal embryos, especially at the PN formation and deletion stage was observed (p<0.05).

Conclusions

As a result, it has been understood that the age of men and women is a factor in the development of aneuploidy; testicular and ejaculate sperm develop aneuploidy embryos at similar rates, time lapse parameters show different processes in the two types of sperm samples, but there are no different rates in terms of aneuploidy, therefore, with this study, it is understood that new morphokinetic models are needed.

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辅助生殖技术周期中受精精子特性与胚胎形态动力学参数和非整倍体可能关系的研究

目的研究和评价不同精子来源胚胎的非整倍体状态及其形态动力学关系。方法对睾丸和射精精子形成的胚胎进行形态动力学评价。根据射精和睾丸精子的使用情况，回顾性评价2016 - 2020年在baheci IVF中心就诊的155例有PGD指征的1157个囊胚的形态动力学和PGD结果。结果在形态动力学评价中，睾丸精子发育短于射精精子发育（p < 0.05）。在35岁前后的评估中发现，女性年龄对同时使用射精和睾丸精子的患者的非整倍体胚胎发育有影响（p<0.05）。PGD分析显示，正常胚胎和异常胚胎的时间流逝过程都比正常胚胎长，特别是在PN形成和缺失阶段（p<0.05）。结论男性和女性的年龄是非整倍体发生的一个因素；睾丸精子和射精精子发育非整倍体胚胎的速率相似，时间推移参数在两种精子样本中显示出不同的过程，但在非整倍体方面没有不同的速率，因此，通过本研究，可以理解需要新的形态动力学模型。

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来源期刊

Reproductive biomedicine online 医学-妇产科学

CiteScore

7.20

自引率

7.50%

发文量

391

审稿时长

50 days

期刊介绍： Reproductive BioMedicine Online covers the formation, growth and differentiation of the human embryo. It is intended to bring to public attention new research on biological and clinical research on human reproduction and the human embryo including relevant studies on animals. It is published by a group of scientists and clinicians working in these fields of study. Its audience comprises researchers, clinicians, practitioners, academics and patients. Context: The period of human embryonic growth covered is between the formation of the primordial germ cells in the fetus until mid-pregnancy. High quality research on lower animals is included if it helps to clarify the human situation. Studies progressing to birth and later are published if they have a direct bearing on events in the earlier stages of pregnancy.