{"title":"Stimulation of prostaglandin E2 biosynthesis by estradiol in rat kidneys.","authors":"W C Chang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The effect of estradiol administration on renal prostaglandin (PG) E2 biosynthetic activity in rats was studied. A specific radioimmunoassay for PGE2 was developed and applied in the quantitation of PGE2 biosynthesis in kidney. Conversion of exogenous arachidonic acid into PGE2 by renal microsomal fraction was assayed. Formation of PGE2 was linear in fashion up to 5 min incubation at 37 degrees C, and linear in fashion up to 3.5 mg of microsome used as enzyme source. The renal biosynthesis of PGE2 was significantly increased by estradiol treatment.</p>","PeriodicalId":20659,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"33 3","pages":"159-63"},"PeriodicalIF":3.2000,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Prostaglandins, leukotrienes, and essential fatty acids","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The effect of estradiol administration on renal prostaglandin (PG) E2 biosynthetic activity in rats was studied. A specific radioimmunoassay for PGE2 was developed and applied in the quantitation of PGE2 biosynthesis in kidney. Conversion of exogenous arachidonic acid into PGE2 by renal microsomal fraction was assayed. Formation of PGE2 was linear in fashion up to 5 min incubation at 37 degrees C, and linear in fashion up to 3.5 mg of microsome used as enzyme source. The renal biosynthesis of PGE2 was significantly increased by estradiol treatment.
期刊介绍：
The role of lipids, including essential fatty acids and their prostaglandin, leukotriene and other derivatives, is now evident in almost all areas of biomedical science. Cell membrane behaviour and cell signalling in all tissues are highly dependent on the lipid constituents of cells. Prostaglandins, Leukotrienes & Essential Fatty Acids aims to cover all aspects of the roles of lipids in cellular, organ and whole organism function, and places a particular emphasis on human studies. Papers concerning all medical specialties are published. Much of the material is particularly relevant to the development of novel treatments for disease.
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