The effect of an exercise- and passive-induced heat stress on autophagy in young and older males.

IF 2.2 3区 医学 Q3 PHYSIOLOGY American journal of physiology. Regulatory, integrative and comparative physiology Pub Date : 2025-03-01 Epub Date: 2025-02-04 DOI:10.1152/ajpregu.00232.2024
James J McCormick, Kelli E King, Nicholas Goulet, Andres E Carrillo, Naoto Fujii, Tatsuro Amano, Pierre Boulay, Glen P Kenny
{"title":"The effect of an exercise- and passive-induced heat stress on autophagy in young and older males.","authors":"James J McCormick, Kelli E King, Nicholas Goulet, Andres E Carrillo, Naoto Fujii, Tatsuro Amano, Pierre Boulay, Glen P Kenny","doi":"10.1152/ajpregu.00232.2024","DOIUrl":null,"url":null,"abstract":"<p><p>Although activation of autophagy is vital for cellular survival during exposure to ambient heat and exercise, it remains unclear if autophagic activity differs between these heat stress conditions and if aging mediates this response. Young [<i>n</i> = 10, mean (SD): 22 (2) yr] and older males [<i>n</i> = 10, 70 (5) yr] performed 30 min of semi-recumbent cycling (70% maximal oxygen uptake). On a separate day, participants were immersed in warm water for 30 min, with the water temperature adjusted to induce the same increase in core temperature (rectal) as the prior exercise bout. Proteins associated with autophagy, inflammation, apoptosis, and the heat shock response (HSR) were assessed in peripheral blood mononuclear cells via Western blot before and after each exposure and during a 6-h seated recovery in a temperate environment (∼22°C). No differences in core temperature occurred at end-exposure to exercise or passive heating in either group (both, <i>P</i> ≥ 0.999). Older adults exhibited greater autophagic regulation (significant LC3-II accumulation) to exercise when compared with passive heating at all time points (all, <i>P</i> ≤ 0.022). However, passive heating alone may have impaired autophagy (elevated p62; <i>P</i> = 0.044). Pro-inflammatory IL-6 was elevated during both conditions (<i>P</i> < 0.001) in older adults. Conversely, greater autophagic initiation (i.e., beclin-2) occurred in young adults at end-exercise and 3-h recovery when compared with passive heating (both, <i>P</i> ≤ 0.024). The HSR and apoptotic responses were similar between conditions in both groups. Although brief exercise stimulates autophagy, exposure to ambient heat stress of an equivalent heat load may underlie autophagic dysregulation in older adults.<b>NEW & NOTEWORTHY</b> We show that a short-duration (30-min) bout of vigorous-intensity exercise stimulates autophagy in young and older males when performed in a temperate environment. However, when exposed to an equivalent heat load as achieved during the prior exercise bout to elicit the same relative increase in core temperature via warm-water immersion, autophagic dysregulation occurs in older but not younger males.</p>","PeriodicalId":7630,"journal":{"name":"American journal of physiology. Regulatory, integrative and comparative physiology","volume":" ","pages":"R289-R299"},"PeriodicalIF":2.2000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of physiology. Regulatory, integrative and comparative physiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1152/ajpregu.00232.2024","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/4 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"PHYSIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Although activation of autophagy is vital for cellular survival during exposure to ambient heat and exercise, it remains unclear if autophagic activity differs between these heat stress conditions and if aging mediates this response. Young [n = 10, mean (SD): 22 (2) yr] and older males [n = 10, 70 (5) yr] performed 30 min of semi-recumbent cycling (70% maximal oxygen uptake). On a separate day, participants were immersed in warm water for 30 min, with the water temperature adjusted to induce the same increase in core temperature (rectal) as the prior exercise bout. Proteins associated with autophagy, inflammation, apoptosis, and the heat shock response (HSR) were assessed in peripheral blood mononuclear cells via Western blot before and after each exposure and during a 6-h seated recovery in a temperate environment (∼22°C). No differences in core temperature occurred at end-exposure to exercise or passive heating in either group (both, P ≥ 0.999). Older adults exhibited greater autophagic regulation (significant LC3-II accumulation) to exercise when compared with passive heating at all time points (all, P ≤ 0.022). However, passive heating alone may have impaired autophagy (elevated p62; P = 0.044). Pro-inflammatory IL-6 was elevated during both conditions (P < 0.001) in older adults. Conversely, greater autophagic initiation (i.e., beclin-2) occurred in young adults at end-exercise and 3-h recovery when compared with passive heating (both, P ≤ 0.024). The HSR and apoptotic responses were similar between conditions in both groups. Although brief exercise stimulates autophagy, exposure to ambient heat stress of an equivalent heat load may underlie autophagic dysregulation in older adults.NEW & NOTEWORTHY We show that a short-duration (30-min) bout of vigorous-intensity exercise stimulates autophagy in young and older males when performed in a temperate environment. However, when exposed to an equivalent heat load as achieved during the prior exercise bout to elicit the same relative increase in core temperature via warm-water immersion, autophagic dysregulation occurs in older but not younger males.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
运动和被动引起的热应激对年轻男性和老年男性自噬的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
5.30
自引率
3.60%
发文量
145
审稿时长
2 months
期刊介绍: The American Journal of Physiology-Regulatory, Integrative and Comparative Physiology publishes original investigations that illuminate normal or abnormal regulation and integration of physiological mechanisms at all levels of biological organization, ranging from molecules to humans, including clinical investigations. Major areas of emphasis include regulation in genetically modified animals; model organisms; development and tissue plasticity; neurohumoral control of circulation and hypertension; local control of circulation; cardiac and renal integration; thirst and volume, electrolyte homeostasis; glucose homeostasis and energy balance; appetite and obesity; inflammation and cytokines; integrative physiology of pregnancy-parturition-lactation; and thermoregulation and adaptations to exercise and environmental stress.
期刊最新文献
Knockdown of the type 1 cannabinoid receptor in the central amygdala increases both spontaneous and water deprivation-induced sodium intake in rats. Heat-producing thermoeffector plasticity in response to prolonged iterative exposure to a high-heat loss environment: no indication of thermoregulatory fatigue. Editorial Focus: "Endothelial dysfunction in middle-aged and older men with low testosterone is associated with elevated circulating endothelin-1". Mechanisms involved in cardiovascular and hydroelectrolytic changes in dehydrated high-fat diet-fed rats. Augmented analgesic effect of tyramine or octopamine in combination with lidocaine is mediated with α-adrenergic receptors.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1