Guidelines for establishing models of immunomodulatory in Caenorhabditis elegans

Abstract

Caenorhabditis elegans is a popular model for studying the interaction between bacterial pathogens, viruses, fungi, and the innate immune system of metazoans. When exposed to a pathogenic organism, a clear response is elicited in the C. elegans which is characterized by specific alterations on the transcriptional and translational levels. In this guideline, we describe the current use of pathogens as media to infect C. elegans and study host immune regulation, and elaborate on how to accurately establish pathogenic models of innate immunity and host–pathogen interactions, which is conducive to researchers to establish corresponding models and effective killing methods according to their own research directions, and then conduct more in-depth research on immune regulatory mechanisms at biological and molecular levels.