Behavioral and histological study on the neuroprotective effect of thymoquinone on the cerebellum in AlCl3-induced neurotoxicity in rats through modulation of oxidative stress, apoptosis, and autophagy.
Amira I Shrief, Dina S Elshenawy, Ahmed E Elsukary, Sally A Elekhtiar, Ola A Yahia
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引用次数: 0
Abstract
Alzheimer disease (AD) is a neurodegenerative condition. Thymoquinone (TQ) is a natural compound that possesses beneficial biological effects on the brain. The present study evaluates the protective impact of TQ on the cerebellum in rats with AlCl3-induced Alzheimer's disease. Four groups were utilized. Control: 20 rats that were subdivided into two subgroups. Ia: received distilled water for 4 weeks. Ib: received corn oil via oral gavage (1 ml/kg daily) for 4 weeks. TQ group: 10 rats received TQ in corn oil via oral gavage (20 mg/kg daily) for 4 weeks. AD group:10 rats received AlCl3 in distilled water via oral gavage (300 mg/kg daily) for 4 weeks. AD & TQ group: 10 rats received both AlCl3 & TQ for 4 weeks. The grip period in the rotarod test decreased, escape latency in first three days and the entry latency period to the quadrant with the removed escape platform in the Morris water maze test increased in AD group, but when TQ was administered concurrently, there was a noteworthy improvement. Meanwhile, when compared to AD group, the addition of TQ showed a significant decrease (P < 0.05) in levels of malondialdehyde (MDA) and nitric oxide (NO), associated with a significant increase (P < 0.05) in reduced glutathione (GSH) level. Furthermore, AD & TQ group exhibited substantial preservation of the cerebellum's histological structure, the Purkinje cells number and transverse diameter showed a high significant increase (P < 0.001) and a significant increase (P < 0.05), respectively in comparison to the AD group. Using TQ showed improvement in behavioral tests, biochemical and histological findings. Thus, TQ might have therapeutic effects on Alzheimer's disease.
期刊介绍：
The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes.
Major research themes of particular interest include:
- Cell-Cell and Cell-Matrix Interactions;
- Connective Tissues;
- Development and Disease;
- Neuroscience.
Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance.
The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.
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