An adhesive and self-healing ROS-scavenging hydrogel loading with hMSC-derived exosomes for diabetic wound healing

Abstract

Diabetic wounds have garnered significant attention due to excessive reactive oxygen species (ROS), persistent inflammation, and vascular and neural impairments that hinder effective healing. ROS-scavenging hydrogels with phenylborate bonds possess inherent anti-ROS and anti-inflammatory properties, while human mesenchymal stem cell-derived exosomes (hMSC-exos) offer additional anti-inflammatory, pro-angiogenic, and neurogenic benefits, presenting a promising strategy to address these challenges. This study introduces a novel ROS-scavenging hydrogel loaded with hMSC-exos, which exhibits strong adhesion and self-healing capabilities. Upon application to the wound, it interacts with ROS to produce an anti-inflammatory response, concurrently allowing a sustained release of hMSC-exos. In vitro and in vivo experiments have demonstrated that this hydrogel effectively reduces ROS levels, mitigates inflammation, and promotes angiogenesis and neurogenesis, thus enhancing functional skin restoration and accelerating wound healing. In summary, we propose an innovative therapeutic approach for diabetic wound healing by combining ROS-scavenging hydrogels with hMSC-exos, with the potential to significantly benefit patients.