Effects of poly(amidoamine) as an extrafibrillar demineralization agent on dentin bonding durability of deciduous teeth

IF 5.5 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of dentistry Pub Date : 2025-03-01 Epub Date: 2025-02-03 DOI:10.1016/j.jdent.2025.105609

Lingling Yuan , Yan Liu , Kunyu Lv, Mengdan Zhang, Xiaoli Hu

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Abstract

Objectives

To investigate the effects of the sixth generation of poly(amidoamine) (G6-PAMAM)-based extrafibrillar demineralization on bonding durability of deciduous dentin and explore the possible mechanisms.

Methods

The cytotoxicity and inhibitory effects on recombinant human matrix metalloproteinases-9 (rhMMP-9) of G6-PAMAM were investigated. The chelation demineralization capacity of G6-PAMAM with molecular weight > 40 kDa was quantitatively analyzed. Atomic force microscopy-infrared spectroscopy (AFM-IR) was used to verify selective extrafibrillar demineralization of dentin conditioned by G6-PAMAM. After dentin surfaces were conditioned with G6-PAMAM or phosphoric acid (H₃PO₄), G6-PAMAM- and H₃PO₄-conditioned dentin were applied with adhesive restoration using both wet- and dry-bonding technique. Microtensile bond strength (μTBS) was evaluated after 24 h storage or 10,000 thermocycling. Nanoleakage expression at the bonding interface was observed using field emission scanning electron microscopy. Gelatinolytic activity within the hybrid layer was examined using in situ zymography.

Results

In addition to being nontoxic, 20 µg/mL G6-PAMAM showed inhibitory effects on rhMMP-9 and calcium-chelating capability. AFM-IR confirmed that G6-PAMAM conditioning can achieve selective demineralization of dentin extrafibrillar minerals. Deciduous dentin treated with 20 µg/mL G6-PAMAM for 60 s produced μTBS equivalent to H₃PO₄-based etch-and-rinse technique. Those bond strengths were maintained after thermocycling, irrespective of wet-bonding or dry-bonding. G6-PAMAM conditioning produced less nanoleakage and suppressed endogenous gelatinolytic activity compare with H₃PO₄ etching.

Conclusion

The G6-PAMAM-based extrafibrillar demineralization strategy under dry-bonding technique could enhance bonding durability of deciduous dentin by retaining intrafibrillar minerals, decreasing interfacial nanoleakage, and preventing endogenous protease-initiated collagen degradation.

Clinical relevance

The G6-PAMAM-based extrafibrillar demineralization strategy has the potential to improve the stability of the resin-dentin bonding interface and prolong the longevity of resin restorations in deciduous teeth.

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聚胺胺作为纤维外脱矿剂对乳牙牙本质结合耐久性的影响。

目的：研究第六代聚胺胺（G6-PAMAM）基纤维外脱矿剂对乳牙本质黏结耐久性的影响，并探讨其可能的机制。方法：研究G6-PAMAM对重组人基质金属蛋白酶-9 （rhMMP-9）的细胞毒性和抑制作用。定量分析了分子量为bbb40 kDa的G6-PAMAM的螯合脱矿能力。采用原子力显微镜-红外光谱（AFM-IR）验证了G6-PAMAM条件下牙本质的选择性纤维外脱矿。牙本质表面用G6-PAMAM或磷酸（H3PO4）处理后，采用干湿粘接技术对G6-PAMAM和H3PO4处理后的牙本质进行粘接修复。储存24 h或热循环10000次后，测定微拉伸键强度（μTBS）。利用场发射扫描电镜观察了键合界面的纳米渗漏表达。利用原位酶谱法检测杂交层内的明胶水解活性。结果：20µg/mL G6-PAMAM除无毒外，还对rhMMP-9和钙螯合能力有抑制作用。AFM-IR证实G6-PAMAM调理可以实现牙本质纤维外矿物的选择性脱矿。用20µg/mL G6-PAMAM处理60 s的乳牙本质产生相当于h3po4基蚀刻冲洗技术的μTBS。无论湿键合还是干键合，这些键合强度在热循环后都保持不变。与H3PO4蚀刻相比，G6-PAMAM处理产生的纳米泄漏较少，内源性明胶溶解活性受到抑制。结论：基于g6 - pamam的纤维外脱矿策略通过保留纤维内矿物质，减少界面纳米渗漏，防止内源性蛋白酶引发的胶原降解，提高了乳牙本质的粘接耐久性。临床意义：以g6 - pamam为基础的纤维外脱矿策略具有提高树脂-牙本质结合界面稳定性和延长树脂修复体在乳牙中的使用寿命的潜力。

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来源期刊

Journal of dentistry 医学-牙科与口腔外科

CiteScore

7.30

自引率

11.40%

发文量

349

审稿时长

35 days

期刊介绍： The Journal of Dentistry has an open access mirror journal The Journal of Dentistry: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal of Dentistry is the leading international dental journal within the field of Restorative Dentistry. Placing an emphasis on publishing novel and high-quality research papers, the Journal aims to influence the practice of dentistry at clinician, research, industry and policy-maker level on an international basis. Topics covered include the management of dental disease, periodontology, endodontology, operative dentistry, fixed and removable prosthodontics, dental biomaterials science, long-term clinical trials including epidemiology and oral health, technology transfer of new scientific instrumentation or procedures, as well as clinically relevant oral biology and translational research. The Journal of Dentistry will publish original scientific research papers including short communications. It is also interested in publishing review articles and leaders in themed areas which will be linked to new scientific research. Conference proceedings are also welcome and expressions of interest should be communicated to the Editor.