Koji Goto, Shigeo Hayashi, Yasuaki Shirayoshi, Masatoshi Takeichi, Hisato Kondoh
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引用次数: 22
Abstract
We developed an experimental system in which differentiation of teratocarcinoma stem cell is probed by expression of stably introduced exogenous genes. We used chicken δ-crystallin gene (δ gene) and its derivative (Moδ gene) driven by long terminal repeat (LTR) promoter of Moloney murine leukemia virus (Mo-MuLV). Neither of the genes was expressed in the undifferentiated condition. Differentiation to primitive endoderm induced by retinoic acid (RA) led to expression of δ but not Moδ, while differentiation to more advanced endodermal cells by RA plus dibutyryl cAMP elicited Moδ expression in addition to δ. These results are interpreted as a consequence of differential activation/suppression of gene expression through enhancer elements associated with the genes.
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