{"title":"Lectin activity and distribution of chicken lactose lectin I in the extracellular matrix of the chick developing kidney","authors":"Eliane Didier , Pierre Didier , Danielle Bayle , Michelle Chevalier","doi":"10.1016/0045-6039(88)90060-7","DOIUrl":null,"url":null,"abstract":"<div><p>A lectin activity inhibitable by thiodigalactose, <em>N</em>-acetyllactosamine, lactulose, lactose and by an antibody raised against CLL I (chicken-lactose lectin I) has been investigated in the chick embryo developing kidney. At post-induction stages this activity was found in both mesonephros and metanephros. In immunofluorescence and immunoelectron microscopy, the extracellular distribution of CLL I was similar in the mesonephros and the metanephros. The lectin was never found intracellularly; cultured kidney cells did not express any endogenous lectin but were rich in lectin-receptor sites, which led to the hypothesis that CLL I is not produced in situ but could be adsorbed on renal cells. Potential physiological roles for embryonic lectins are discussed.</p></div>","PeriodicalId":75684,"journal":{"name":"Cell differentiation","volume":"24 2","pages":"Pages 83-95"},"PeriodicalIF":0.0000,"publicationDate":"1988-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0045-6039(88)90060-7","citationCount":"13","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell differentiation","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0045603988900607","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 13
Abstract
A lectin activity inhibitable by thiodigalactose, N-acetyllactosamine, lactulose, lactose and by an antibody raised against CLL I (chicken-lactose lectin I) has been investigated in the chick embryo developing kidney. At post-induction stages this activity was found in both mesonephros and metanephros. In immunofluorescence and immunoelectron microscopy, the extracellular distribution of CLL I was similar in the mesonephros and the metanephros. The lectin was never found intracellularly; cultured kidney cells did not express any endogenous lectin but were rich in lectin-receptor sites, which led to the hypothesis that CLL I is not produced in situ but could be adsorbed on renal cells. Potential physiological roles for embryonic lectins are discussed.
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