Roles of B-cell lymphoma 6 in orthodontic tooth movement of rat molars.

IF 2.7 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE European journal of orthodontics Pub Date : 2025-02-07 DOI:10.1093/ejo/cjaf006

Wasupol Sodsook, Yukiho Kobayashi, Hiroyuki Kamimoto, Yuki Niki, Koji Yokoo, Pintu-On Chantarawaratit, Keiji Moriyama

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Abstract

Introduction: B-cell lymphoma 6 (Bcl6) inhibits osteoclast differentiation in vitro; however, its role in orthodontic tooth movement (OTM) remains unclear. This study aimed to investigate the role of Bcl6 in OTM of rat molars.

Materials and methods: OTM was performed on the maxillary first molars of male rats using nickel-titanium coil springs (25 gf) for 14 days with or without local injection of FX1 (50 mg/kg), a Bcl6 inhibitor (n = 10 per group). Micro-computed tomography (CT) images were used to analyse OTM distance and bone morphometric parameters. Immunohistochemistry (IHC) determined Bcl6 expression and tartrate-resistant acid phosphatase staining (TRAP) staining assessed osteoclast differentiation. TRAP staining, and reverse transcription-quantitative polymerase chain reaction determined the effect of FX1 (1 μM) on in vitro rat osteoclast differentiation. The effect of FX1 on cell proliferation and Smad4 expression in periodontal ligament (PDL) cells was determined.

Results: Administration of FX1 significantly increased OTM distance and decreased the bone/tissue volume compared with vehicle treatment. IHC staining showed that the vehicle-OTM group had higher expression of Bcl6 than the FX1-OTM group. The number of osteoclasts on the compression side was significantly higher in the FX1-OTM group than that in the vehicle-OTM group. FX1 enhanced osteoclast differentiation and expression of Nfatc1, Dc-stamp, and Ctsk mRNA in osteoclasts in vitro. FX1 significantly promotes PDL cell proliferation in vivo and in vitro.

Limitations: We evaluated only 14 days of OTM.

Conclusions: Bcl6 may play an important role in OTM via modulation of osteoclast differentiation and PDL cell proliferation.

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b细胞淋巴瘤6在大鼠磨牙正畸牙齿运动中的作用。

b细胞淋巴瘤6 （Bcl6）体外抑制破骨细胞分化；然而，其在正畸牙齿运动（OTM）中的作用尚不清楚。本研究旨在探讨Bcl6在大鼠磨牙OTM中的作用。材料与方法：采用镍钛线圈弹簧（25 gf）对雄性大鼠上颌第一磨牙进行OTM，局部注射或不局部注射Bcl6抑制剂FX1 (50 mg/kg)，每组10只。显微计算机断层扫描（CT）图像分析OTM距离和骨形态测量参数。免疫组化（IHC）检测Bcl6表达，抗酒石酸酸性磷酸酶（TRAP）染色评估破骨细胞分化。TRAP染色，逆转录-定量聚合酶链反应测定FX1 （1 μM）对体外大鼠破骨细胞分化的影响。观察FX1对牙周韧带（PDL）细胞增殖及Smad4表达的影响。结果：与载药治疗相比，FX1显著增加了骨外膜距离，减少了骨/组织体积。免疫组化染色显示，载药- otm组Bcl6表达高于FX1-OTM组。FX1-OTM组压迫侧破骨细胞数量明显高于vehicle-OTM组。FX1增强体外破骨细胞的分化和Nfatc1、Dc-stamp、Ctsk mRNA的表达。FX1在体内外均显著促进PDL细胞增殖。局限性：我们只评估了14天的OTM。结论：Bcl6可能通过调节破骨细胞分化和PDL细胞增殖在OTM中发挥重要作用。

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来源期刊

European journal of orthodontics 医学-牙科与口腔外科

CiteScore

5.50

自引率

7.70%

发文量

审稿时长

4-8 weeks

期刊介绍： The European Journal of Orthodontics publishes papers of excellence on all aspects of orthodontics including craniofacial development and growth. The emphasis of the journal is on full research papers. Succinct and carefully prepared papers are favoured in terms of impact as well as readability.