A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types.

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS STAR Protocols Pub Date : 2025-03-21 Epub Date: 2025-02-05 DOI:10.1016/j.xpro.2025.103619
Lakshmi Jayaram, Ramith Ramu, Deepthi Puttegowda, Jayanthi M K, Chandana Kumari V B, Khang Wen Goh
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引用次数: 0

Abstract

Lactic acid bacteria (LAB) are a large group produced during carbohydrate fermentation, resulting in lactic acid. Here, we present a protocol for probiotic adhesion and interaction into buccal epithelial cells, chicken epithelial cells, and HT-29 cells, as well as for autoaggregation and coaggregation. We describe steps for visualizing LAB adhesion by imaging cell adhesion using a light microscope. We then detail procedures for performing an adhesion assay. For complete details on the use and execution of this protocol, please refer to Kumari et al.1.

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一种评估乳酸菌对不同细胞类型的粘附潜力的方案。
乳酸菌(LAB)是在碳水化合物发酵过程中产生的一大群细菌,产生乳酸。在这里,我们提出了一种益生菌粘附和相互作用到口腔上皮细胞、鸡上皮细胞和HT-29细胞以及自聚集和共聚集的方案。我们描述了通过光学显微镜成像细胞粘附来可视化LAB粘附的步骤。然后我们详细介绍了进行粘附试验的程序。有关本协议使用和执行的完整细节,请参考Kumari等人1。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
STAR Protocols
STAR Protocols Biochemistry, Genetics and Molecular Biology-General Biochemistry, Genetics and Molecular Biology
CiteScore
2.00
自引率
0.00%
发文量
789
审稿时长
10 weeks
期刊介绍:
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