Spatial transcriptomics defines the cell-specific RNA landscape of equine dorsal root ganglia.

Abstract

Equine spinal neurodegenerative conditions are frequently encountered in sport and racing horses and may be career-ending diagnoses. To further define the spatial transcriptomic landscape of equine dorsal root ganglia (DRG) in healthy adult horses, we investigated gene expression differences in distinct DRG regions using the GeoMx Digital Spatial Profiling from NanoString. Four human cell markers demonstrated high fidelity for equine cells; microtubule-associated protein 2 (MAP2), myelin basic protein (MBP), allograft inflammatory 104 factor 1/ionized calcium-binding adaptor molecule 1 (IBA1/AIF1), and Syto83 nuclear marker. Geometric regions of interest were then selected as MBP-rich, IBA1-high, and IBA1-low, and gene expression was compared. Experimental validation was achieved, with genes involved in myelination enriched in MBP-rich regions, and the identification of glia-specific genes enriched in IBA1-high regions. Thus, spatial transcriptomics with human cell markers was successful in equine DRG and can now be applied to determine cell-specific transcriptional changes during disease states.