Characterization, expression and interactive proteins analysis of BjuB022815 in B. juncea

Abstract

Brassica juncea is a vegetative vegetable, and its yield and quality can be significantly affected by flowering time. In this study, BjuB022815 with other 33 RLKs gene were further divided into 5 subfamilies, most of which contained protein kinase C-like (PKc-like), Malectin-like and STkc-IRAK domains. BjuB022815, which was significantly upregulated at the reproductive stage, was chosen for further study. To explore the interactions of these proteins, Y2HGold assays were conducted with BjuB022815 as bait and four proteins as preys. Y2H and BiFC (Bimolecular florescence) assays confirmed that BjuB022815 interacted with BjuA018253 and BjuVA09G13910. qRT-PCR revealed that BjuB022815 was significantly upregulated in flower buds and flowers. At the developmental stage, BjuB022815 and BjuA018253 were significantly upregulated in the bolting stage, while BjuVA09G13910 was downregulated in the reproductive stages. Subcellular assays revealed that BjuB022815, BjuA018253 and BjuVA09G13910 were located in the nucleus. Based on these results, it speculated that BjuB022815 and BjuA018253 interact in the nucleus and function in flowering time regulation in B. juncea.