Mingfeng Lai, Shitao Lu, Bao Huang, Yanfeng Nie, Xu Wang, Jiaying Xiong and Zhenning Yu
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引用次数: 0
Abstract
We developed a PCR-free, cost-effective colorimetric assay using gold nanoparticles (AuNPs) to quantify 5-hydroxymethylcytosine (5hmC) without expensive MS instruments or antibodies. This method employs a photo-elutable 8–17 DNAzyme label specific to 5hmC sites, achieving accuracy comparable to ELISA when measuring 5hmC levels in various ICR mouse tissues.
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