Hypericum Perforatum-Derived Exosomes-Like Nanovesicles: A Novel Natural Photosensitizer for Effective Tumor Photodynamic Therapy.

IF 6.5 2区医学 Q1 NANOSCIENCE & NANOTECHNOLOGY International Journal of Nanomedicine Pub Date : 2025-02-04 eCollection Date: 2025-01-01 DOI:10.2147/IJN.S510339

Xiaoyu Ma, Ni Chen, Peiyuan Zeng, Yuqian He, Tao Zhang, Yu Lu, Ziyu Li, Jin Xu, Jingcan You, Youkun Zheng, Liqun Wang, Mao Luo, Jianbo Wu

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Abstract

Background: Natural photosensitizers hold potential for photodynamic therapy (PDT) but are often limited by poor visible light absorption. Plant-derived exosome-like nanovesicles offer an innovative platform for enhancing photosensitizer performance.

Methods: Hypericum perforatum-derived nanovesicles (HPDENs) were characterized using electron microscopy, dynamic light scattering, and proteomic and miRNA sequencing. High-performance liquid chromatography confirmed hypericin content. PDT efficacy was assessed in vitro and in vivo.

Results: HPDENs exhibited robust photosensitizing properties, generating reactive oxygen species (ROS) through both Type I and Type II pathways upon light activation. In vitro, HPDENs showed light dose-dependent cytotoxicity against human melanoma cells, characterized by elevated ROS production and apoptosis induction. In vivo, HPDEN-mediated PDT significantly suppressed tumor growth and induced extensive tumor necrosis, with no observable toxicity to major organs.

Conclusion: HPDENs represent a novel plant-derived photosensitizer with dual ROS generation pathways and significant therapeutic efficacy, providing a promising platform for enhancing photodynamic therapy.

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穿孔金丝桃衍生的外泌体样纳米囊泡：一种用于有效肿瘤光动力治疗的新型天然光敏剂。

背景：天然光敏剂具有光动力治疗（PDT）的潜力，但通常受到可见光吸收差的限制。植物衍生的外泌体样纳米囊泡为增强光敏剂性能提供了一个创新的平台。方法：利用电子显微镜、动态光散射、蛋白质组学和miRNA测序对穿孔金丝桃衍生的纳米囊泡（hptens）进行表征。高效液相色谱法证实金丝桃素含量。评估PDT在体内和体外的疗效。结果：HPDENs表现出强大的光敏特性，在光激活后通过I型和II型途径产生活性氧（ROS）。在体外，hptens对人黑色素瘤细胞表现出轻度剂量依赖性的细胞毒性，其特征是ROS产生升高和诱导细胞凋亡。在体内，hpden介导的PDT显著抑制肿瘤生长并诱导广泛的肿瘤坏死，对主要器官无明显毒性。结论：HPDENs是一种新型的植物源光敏剂，具有双ROS生成途径，具有显著的治疗效果，为加强光动力治疗提供了良好的平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Nanomedicine NANOSCIENCE & NANOTECHNOLOGY-PHARMACOLOGY & PHARMACY

CiteScore

14.40

自引率

3.80%

发文量

511

审稿时长

1.4 months

期刊介绍： The International Journal of Nanomedicine is a globally recognized journal that focuses on the applications of nanotechnology in the biomedical field. It is a peer-reviewed and open-access publication that covers diverse aspects of this rapidly evolving research area. With its strong emphasis on the clinical potential of nanoparticles in disease diagnostics, prevention, and treatment, the journal aims to showcase cutting-edge research and development in the field. Starting from now, the International Journal of Nanomedicine will not accept meta-analyses for publication.