dsRNAEngineer: a web-based tool of comprehensive dsRNA design for pest control.

IF 14.3 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Trends in biotechnology Pub Date : 2025-02-08 DOI:10.1016/j.tibtech.2025.01.002

Yang Chen, Yufei Shi, Ziguo Wang, Xin An, Siyu Wei, Christos Andronis, John Vontas, Jin-Jun Wang, Jinzhi Niu

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引用次数: 0

Abstract

Over the past two decades, many double-stranded (ds)-RNAs have been synthesized to silence target genes for exploration of gene functions in pests. Some of these dsRNAs are lethal to pests, leading to a new category of pesticides. The generation of these environmentally friendly pesticides requires precise in silico design of dsRNA molecules that target pests but not non-pest organisms. Current efforts in dsRNA design focus mainly on the analysis of the target gene sequence, lacking comprehensive analysis of all transcripts of the whole transcriptome per given species, causing low efficiency and imprecise dsRNA target exploration. To address these limitations, we created the dsRNAEngineer online platform (https://dsrna-engineer.cn), which allows comprehensive and rational dsRNA design, incorporating hundreds of pest and non-pest transcriptomes. Developed functionalities include screen-target (screen conserved genes for cotargets of various pest species), on-target, off-target, and multi-target to generate optimal dsRNA for precise pest control.

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来源期刊

Trends in biotechnology 工程技术-生物工程与应用微生物

CiteScore

28.60

自引率

1.20%

发文量

198

审稿时长

1 months

期刊介绍： Trends in Biotechnology publishes reviews and perspectives on the applied biological sciences, focusing on useful science applied to, derived from, or inspired by living systems. The major themes that TIBTECH is interested in include: Bioprocessing (biochemical engineering, applied enzymology, industrial biotechnology, biofuels, metabolic engineering) Omics (genome editing, single-cell technologies, bioinformatics, synthetic biology) Materials and devices (bionanotechnology, biomaterials, diagnostics/imaging/detection, soft robotics, biosensors/bioelectronics) Therapeutics (biofabrication, stem cells, tissue engineering and regenerative medicine, antibodies and other protein drugs, drug delivery) Agroenvironment (environmental engineering, bioremediation, genetically modified crops, sustainable development).

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