dsRNAEngineer: a web-based tool of comprehensive dsRNA design for pest control.

IF 14.9 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Trends in biotechnology Pub Date : 2025-04-01 Epub Date: 2025-02-08 DOI:10.1016/j.tibtech.2025.01.002

Yang Chen, Yufei Shi, Ziguo Wang, Xin An, Siyu Wei, Christos Andronis, John Vontas, Jin-Jun Wang, Jinzhi Niu

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Abstract

Over the past two decades, many double-stranded (ds)-RNAs have been synthesized to silence target genes for exploration of gene functions in pests. Some of these dsRNAs are lethal to pests, leading to a new category of pesticides. The generation of these environmentally friendly pesticides requires precise in silico design of dsRNA molecules that target pests but not non-pest organisms. Current efforts in dsRNA design focus mainly on the analysis of the target gene sequence, lacking comprehensive analysis of all transcripts of the whole transcriptome per given species, causing low efficiency and imprecise dsRNA target exploration. To address these limitations, we created the dsRNAEngineer online platform (https://dsrna-engineer.cn), which allows comprehensive and rational dsRNA design, incorporating hundreds of pest and non-pest transcriptomes. Developed functionalities include screen-target (screen conserved genes for cotargets of various pest species), on-target, off-target, and multi-target to generate optimal dsRNA for precise pest control.

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dsRNAEngineer：一个基于网络的害虫控制综合dsRNA设计工具。

在过去的二十年里，人们合成了许多双链rna来沉默目标基因，以探索害虫的基因功能。其中一些dsrna对害虫是致命的，导致了一类新的杀虫剂。这些环境友好型杀虫剂的产生需要精确的dsRNA分子的硅设计，以害虫为目标，而不是非害虫生物。目前dsRNA的设计工作主要集中在靶基因序列的分析上，缺乏对给定物种的整个转录组的所有转录本的综合分析，导致dsRNA靶点探索效率低，精度不高。为了解决这些限制，我们创建了dsRNAEngineer在线平台（https://dsrna-engineer.cn），该平台允许全面合理的dsRNA设计，包含数百种害虫和非害虫转录组。开发的功能包括筛选靶点（筛选各种害虫物种的共同靶点的保守基因），靶点，非靶点和多靶点，以产生精确控制害虫的最佳dsRNA。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in biotechnology 工程技术-生物工程与应用微生物

CiteScore

28.60

自引率

1.20%

发文量

198

审稿时长

1 months

期刊介绍： Trends in Biotechnology publishes reviews and perspectives on the applied biological sciences, focusing on useful science applied to, derived from, or inspired by living systems. The major themes that TIBTECH is interested in include: Bioprocessing (biochemical engineering, applied enzymology, industrial biotechnology, biofuels, metabolic engineering) Omics (genome editing, single-cell technologies, bioinformatics, synthetic biology) Materials and devices (bionanotechnology, biomaterials, diagnostics/imaging/detection, soft robotics, biosensors/bioelectronics) Therapeutics (biofabrication, stem cells, tissue engineering and regenerative medicine, antibodies and other protein drugs, drug delivery) Agroenvironment (environmental engineering, bioremediation, genetically modified crops, sustainable development).

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