Signal-amplifying biosensors for miRNA detection and their extension in portable glucometer

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL Talanta Pub Date : 2025-02-13 DOI:10.1016/j.talanta.2025.127714

Xiaofang Liu , Qun Wang , Huan Liu , Yong Zhang , Mei Yang , Danqun Huo , Changjun Hou

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Abstract

Timely detection of breast cancer not only positively influences patient treatment and life quality, but also possesses far-reaching implications for public health and medical research. And miRNAs, as emerging breast cancer-related biomarkers, demonstrate heightened sensitivity to disease progression. In this study, the same precursor was utilized to prepare micromaterials with large and small cavities, this was the safeguard for sensing probes and signals. And the signal amplification process was accomplished with strand displacement assistance by target excitation. Encouragingly, it was successfully and accurately detected miRNA-21 and miRNA-155 within 10 fmol/L - 10 nmol/L. Due to the unique sensing design, the signal can be read out not only by small fluorescent devices, but also by portable glucometers. The biosensor features several excellent working performances and illuminates resource-poor areas by equipping the most common glucometers. So, it is a sensitive, portable and promising biosensor.

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.