New nucleopolyhedrovirus isolate in the management of Spodoptera eridania.

IF 1.1 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Anais da Academia Brasileira de Ciencias Pub Date : 2025-02-10 eCollection Date: 2025-01-01 DOI:10.1590/0001-3765202520230077
Alixelhe P Damascena, Fernando Z Madalon, Luis M DE Araujo Junior, Dirceu Pratissoli, Carlos Eduardo C Paiva, Lorena C Machado, Hugo Gonçalves Junior, Vinícius H Bello
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Abstract

Spodoptera eridania is considered a polyphagous pest, as it attacks several crops. The aim of this study was to identify a virus isolate present in symptomatic S. eridania caterpillars. The virus sample was extracted and OBs purification performed. Viral DNA was extracted using the PureLink Viral RNA/DNA Mini Kit (ThermoFisher) and primers prl8-1 and prl8-1B were used for amplification of the gene fragment. The isolate was identified showing high genetic similarity with the isolate "Spodoptera eridania nucleopolyhedrovirus isolate CNPSo-165", characterizing itself as a novelty in the scenario and as promising, adding the tactics available for the integrated management of S. eridania.

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新的核多角体病毒分离株在斑点夜蛾防治中的应用。
黑斑夜蛾被认为是一种多食害虫,因为它攻击几种作物。本研究的目的是鉴定一种存在于有症状的绿脓杆菌毛虫中的病毒分离物。提取病毒样本并进行OBs纯化。使用PureLink病毒RNA/DNA迷你试剂盒(ThermoFisher)提取病毒DNA,用引物pr18 -1和pr18 - 1b扩增基因片段。该分离物与“斑点夜蛾核型多角体病毒分离物CNPSo-165”具有较高的遗传相似性,为斑点夜蛾的综合防治提供了新思路和新方法。
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来源期刊
Anais da Academia Brasileira de Ciencias
Anais da Academia Brasileira de Ciencias 综合性期刊-综合性期刊
CiteScore
2.20
自引率
0.00%
发文量
347
审稿时长
1 months
期刊介绍: The Brazilian Academy of Sciences (BAS) publishes its journal, Annals of the Brazilian Academy of Sciences (AABC, in its Brazilianportuguese acronym ), every 3 months, being the oldest journal in Brazil with conkinuous distribukion, daking back to 1929. This scienkihic journal aims to publish the advances in scienkihic research from both Brazilian and foreigner scienkists, who work in the main research centers in the whole world, always looking for excellence. Essenkially a mulkidisciplinary journal, the AABC cover, with both reviews and original researches, the diverse areas represented in the Academy, such as Biology, Physics, Biomedical Sciences, Chemistry, Agrarian Sciences, Engineering, Mathemakics, Social, Health and Earth Sciences.
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