Establishment and evaluation of a UPLC-MS/MS method for simultaneous determination of bongkrekic acid and dehydroacetic acid in rice noodles.

IF 4.2 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Frontiers in Chemistry Pub Date : 2025-01-28 eCollection Date: 2024-01-01 DOI:10.3389/fchem.2024.1386635

Lingguo Zhao, Ronggui Lv, Jiang Song, Xi Chen, Lei Lei, Weixian Zeng, Miaowen She, Dabing Li, Xiaxia Yu, Zhanguo Liu, Xiao Wang, Yong Liu

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Abstract

A UPLC-MS/MS method with one-step extraction and simultaneous determination of bongkrekic acid (BA) and dehydroacetic acid (DHA) in rice noodles has been established in this study for the first time. The extraction solvent consisted of a mixture of methanol, ammonia, and water. Electrospray ionization negative ion mode (ESI-) was used for multiple reaction detection and external standard method was used for quantitative determination. The method demonstrated a good linearity range of 0-50 ng/mL with a correlation coefficient (r) of 0.9998 for BA, and showed a linearity range of 0∼500 ng/mL with r of 0.9993 for DHA. The limits of detection (LOD) were determined to be 0.1 μg/kg for BA and 0.6 μg/kg for DHA. Additionally, the method exhibited good recovery of 90.1%∼105.4% for BA and 80.4%∼102% for DHA. Meanwhile, the method also exhibited good precision with RSD of 0.4%∼7.5% for BA, and with RSD of 4.5%∼11.4% for DHA. Furthermore, the established method was sucessfully applied to the analysis of rice noodles from two production enterprises. BA was detected in one rice noodle sample and DHA was detected in 7 rice noodle samples in the enterprises A which was the manufacturer of food consumed by the poisoned patient. To investigate the cause of death of the poisoned patient, the toxigenic bacterium Burkholderia gladiolus was isolated and cultured to produce BA. The results showed that the established method could also be used to qualitatively screen BA and its isomer isobongkrekic acid (IBA) in GVC enrichment broth, potato glucose agar and rice sample, which facilitates a rapid and accurate judgement of whether the isolated bacterium strain produces BA. With high sensitivity and accuracy, and simple sample pretreatment, the proposed method shows potential for safety assessment of the entire production process of rice-derived products, addressing a significant public health concern.

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超高效液相色谱-质谱联用法测定米粉中苯丙酸和脱氢乙酸的建立及评价

本研究首次建立了一步提取同时测定米粉中bongkrekacid （BA）和dehydroacetic acid （DHA）的UPLC-MS/MS方法。萃取溶剂由甲醇、氨和水的混合物组成。采用电喷雾电离负离子模式（ESI-）进行多反应检测，采用外标法进行定量测定。BA的线性范围为0 ~ 50 ng/mL，相关系数(r)为0.9998；DHA的线性范围为0 ~ 500 ng/mL，相关系数(r)为0.9993。BA和DHA的检出限分别为0.1 μg/kg和0.6 μg/kg。此外，该方法对BA的回收率为90.1% ~ 105.4%，对DHA的回收率为80.4% ~ 102%。同时，该方法也具有良好的精密度，BA的RSD为0.4% ~ 7.5%，DHA的RSD为4.5% ~ 11.4%。并将所建立的方法成功地应用于两家生产企业的米粉的分析。中毒患者所食用食品的生产企业A企业1份米粉样品中检出BA， 7份米粉样品中检出DHA。为查明中毒患者的死亡原因，分离出产毒细菌剑兰伯克霍尔德菌，培养产生BA。结果表明，所建立的方法也可用于GVC富集肉汤、马铃薯葡萄糖琼脂和大米样品中BA及其异构体IBA的定性筛选，有助于快速准确地判断所分离菌株是否产生BA。该方法灵敏度高，准确度高，样品预处理简单，具有对大米产品整个生产过程进行安全评估的潜力，解决了一个重大的公共卫生问题。

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来源期刊

Frontiers in Chemistry Chemistry-General Chemistry

CiteScore

8.50

自引率

3.60%

发文量

1540

审稿时长

12 weeks

期刊介绍： Frontiers in Chemistry is a high visiblity and quality journal, publishing rigorously peer-reviewed research across the chemical sciences. Field Chief Editor Steve Suib at the University of Connecticut is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to academics, industry leaders and the public worldwide. Chemistry is a branch of science that is linked to all other main fields of research. The omnipresence of Chemistry is apparent in our everyday lives from the electronic devices that we all use to communicate, to foods we eat, to our health and well-being, to the different forms of energy that we use. While there are many subtopics and specialties of Chemistry, the fundamental link in all these areas is how atoms, ions, and molecules come together and come apart in what some have come to call the “dance of life”. All specialty sections of Frontiers in Chemistry are open-access with the goal of publishing outstanding research publications, review articles, commentaries, and ideas about various aspects of Chemistry. The past forms of publication often have specific subdisciplines, most commonly of analytical, inorganic, organic and physical chemistries, but these days those lines and boxes are quite blurry and the silos of those disciplines appear to be eroding. Chemistry is important to both fundamental and applied areas of research and manufacturing, and indeed the outlines of academic versus industrial research are also often artificial. Collaborative research across all specialty areas of Chemistry is highly encouraged and supported as we move forward. These are exciting times and the field of Chemistry is an important and significant contributor to our collective knowledge.