Inhibition of EPSPS overexpression of glyphosate-resistant Lolium perenne L. with a clethodim and glyphosate mixture

Abstract

Glyphosate-resistant populations of some grass species have demonstrated high sensitivity to combinations of glyphosate and acetyl-CoA carboxylase (ACCase)-inhibiting herbicides. Nevertheless, it is unclear how glyphosate contributes to the control of glyphosate-resistant plants when combined with other active ingredients such as clethodim. The current work aimed to detect if glyphosate-resistant Lolium perenne L. biotypes associated with target-site [5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)] overexpression and EPSPS mutation (P106S) as mechanisms of resistance are equally controlled by clethodim or clethodim plus glyphosate compared to a susceptible counterpart.