Enhanced nitrogen removal through bioaugmentation with Stutzerimonas stutzeri SW22: From denitrification mechanism to optimized sequencing batch reactor

IF 6.7 2区工程技术 Q1 ENGINEERING, CHEMICAL Journal of water process engineering Pub Date : 2025-03-01 Epub Date: 2025-02-13 DOI:10.1016/j.jwpe.2025.107252

Yuhang Song , Lingmin Zhao , Lixing Huang , Yingxue Qin , Jiaonan Zhang , Jiaoling Zhang , Qingpi Yan

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Abstract

A novel heterotrophic nitrification-aerobic denitrification strain SW22, identified as Stutzerimonas stutzeri, was isolated from an aquaculture wastewater treatment system. The strain possessed key denitrification genes (amoA, napA, nirS, norB, nosZ) and demonstrated optimal performance at pH 7.5, 30 °C, and C/N ratio 14–18. In real aquaculture effluent, SW22 achieved removal efficiencies of 76.83 %, 75.32 %, and 65.71 % for NH₄⁺-N, NO₂⁻-N and NO₃⁻-N, respectively. A bioaugmented sequencing batch reactor (q-SBR) was developed with optimized parameters through response surface methodology. The q-SBR system significantly enhanced nitrogen removal (90.51 % TN removal) compared to conventional SBR (77.36 %). Microbial community analysis revealed that bioaugmentation promoted the enrichment of denitrifying bacteria, suggesting its potential for aquaculture wastewater treatment.

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Stutzerimonas stutzeri SW22生物强化脱氮：从反硝化机理到优化的序批式反应器

从水产养殖废水处理系统中分离到一株异养硝化-好氧反硝化菌株SW22，鉴定为Stutzerimonas stutzeri。该菌株具有amoA、napA、nirS、norB、nosZ等关键反硝化基因，在pH 7.5、30℃、C/N比值14-18条件下表现最佳。在实际养殖出水中，SW22对NH4+-N、NO2−-N和NO3−-N的去除率分别为76.83%、75.32%和65.71%。利用响应面法优化了q-SBR反应器的工艺参数。与常规SBR（77.36%）相比，q-SBR系统显著提高了氮的去除率（90.51%）。微生物群落分析表明，生物强化可促进反硝化细菌的富集，表明其在水产养殖废水处理中的潜力。

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来源期刊

Journal of water process engineering Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

10.70

自引率

8.60%

发文量

846

审稿时长

24 days

期刊介绍： The Journal of Water Process Engineering aims to publish refereed, high-quality research papers with significant novelty and impact in all areas of the engineering of water and wastewater processing . Papers on advanced and novel treatment processes and technologies are particularly welcome. The Journal considers papers in areas such as nanotechnology and biotechnology applications in water, novel oxidation and separation processes, membrane processes (except those for desalination) , catalytic processes for the removal of water contaminants, sustainable processes, water reuse and recycling, water use and wastewater minimization, integrated/hybrid technology, process modeling of water treatment and novel treatment processes. Submissions on the subject of adsorbents, including standard measurements of adsorption kinetics and equilibrium will only be considered if there is a genuine case for novelty and contribution, for example highly novel, sustainable adsorbents and their use: papers on activated carbon-type materials derived from natural matter, or surfactant-modified clays and related minerals, would not fulfil this criterion. The Journal particularly welcomes contributions involving environmentally, economically and socially sustainable technology for water treatment, including those which are energy-efficient, with minimal or no chemical consumption, and capable of water recycling and reuse that minimizes the direct disposal of wastewater to the aquatic environment. Papers that describe novel ideas for solving issues related to water quality and availability are also welcome, as are those that show the transfer of techniques from other disciplines. The Journal will consider papers dealing with processes for various water matrices including drinking water (except desalination), domestic, urban and industrial wastewaters, in addition to their residues. It is expected that the journal will be of particular relevance to chemical and process engineers working in the field. The Journal welcomes Full Text papers, Short Communications, State-of-the-Art Reviews and Letters to Editors and Case Studies