Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napus.

IF 6.9 1区生物学 Q1 CELL BIOLOGY Cell reports Pub Date : 2025-02-25 Epub Date: 2025-02-11 DOI:10.1016/j.celrep.2025.115280

Jiang Ye, Xiaowei Wu, Xiang Li, Yuting Zhang, Yuqing Li, Hui Zhang, Jie Chen, Yuyan Xiang, Yefan Xia, Hu Zhao, Zengdong Tan, Liang Guo, Xuan Yao

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Abstract

Seed oil is synthesized in the embryo, which is surrounded by the seed coat. However, the genetic and molecular mechanisms of how seed coat development affects seed oil content (SOC) remains less studied. Through genome-wide association studies in Brassica napus accessions, we identify BnaA09.MYB52 transcription factor as a candidate gene underlying ZS11_qSCC.A09. Knocking out BnaMYB52 results in a 7.7%-8.2% reduction in seed coat content (SCC) but a 12.3%-13.4% increase in SOC, whereas overexpression of BnaA09.MYB52 leads to the opposite effects. Intriguingly, BnaA09.MYB52 has predominant expression in seed coat during seed development. BnaA09.MYB52 activates the expression of PMEI14 and BAN. Mutant analyses indicate that the SOC is increased by 4.3%-7.7%, while seed coat thickness shows a 12.9%-22.8% reduction in the ban and pmei14 mutants. Thus, our findings identify the molecular mechanism of MYB52-BAN/PMEI14 modules regulating SCC and SOC and provide a potential target for oil content improvement in B. napus.

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通过调控BnaMYB52调控甘蓝型油菜种皮含量提高含油量。

种子油是在被种皮包围的胚胎中合成的。然而，种皮发育对种子含油量影响的遗传和分子机制研究较少。通过对甘蓝型油菜的全基因组关联研究，我们鉴定出了BnaA09。MYB52转录因子作为ZS11_qSCC.A09的候选基因。BnaMYB52基因的缺失导致种皮含量（SCC）降低7.7% ~ 8.2%，SOC增加12.3% ~ 13.4%，而BnaA09基因的过表达导致SCC降低7.7% ~ 8.2%。MYB52会产生相反的效果。有趣的是,BnaA09。MYB52在种子发育过程中主要表达于种皮。BnaA09。MYB52激活PMEI14和BAN的表达。突变体分析表明，ban和pmei14突变体的SOC增加了4.3% ~ 7.7%，种皮厚度减少了12.9% ~ 22.8%。因此，我们的研究结果确定了MYB52-BAN/PMEI14模块调节SCC和SOC的分子机制，并为油菜含油量的改善提供了潜在的靶点。

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.