Unveiling the prognostic significance of RNA editing-related genes in colon cancer: evidence from bioinformatics and experiment.

IF 3.4 3区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL European Journal of Medical Research Pub Date : 2025-02-12 DOI:10.1186/s40001-025-02335-7

Zhengcong Deng, Xueqin Jin, Bingxue Liu, Hongyan Zhen, Xiang Wang

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Abstract

Background: RNA editing is recognized as a crucial factor in cancer biology. Its potential application in predicting the prognosis of colon adenocarcinoma (COAD) remains unexplored.

Methods: RNA editing data of COAD patients were downloaded from the Synapse database. LASSO regression was used to construct the risk model and verified by the receiver operating characteristic (ROC) curve. GO and KEGG enrichment analyses were performed to delineate the biological significance of the differentially expressed genes. Finally, differential analysis and immunohistochemistry were used to verify the expression of adenosine deaminase 1 (ADAR1).

Results: We evaluated a total of 4079 RNA editing sites in 514 COAD patients from Synapse database. A prognostic signature was constructed based on five genes were significantly associated with the prognosis of COAD patients including GNL3L, NUP43, MAGT1, EMP2, and ARSD. Univariate and multivariate Cox regression analysis revealed that RNA editing-related genes (RERGs)-related signature was an independent risk factor for COAD. Moreover, Experimental evidence shows that ADAR1 is highly expressed in colon adenocarcinoma and silencing ADAR1 can inhibit cancer cell proliferation.

Conclusion: We established a prognostic model based on five RERGs with strong predictive value. This model not only serves as a foundation for a novel prognostic tool but also facilitates the identification of potential drug candidates for treating COAD.

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揭示RNA编辑相关基因在结肠癌中的预后意义：来自生物信息学和实验的证据。

背景：RNA编辑被认为是癌症生物学中的一个关键因素。它在预测结肠腺癌（COAD）预后方面的潜在应用仍未探索。方法：从Synapse数据库下载COAD患者的RNA编辑数据。采用LASSO回归构建风险模型，并通过受试者工作特征（ROC）曲线进行验证。进行GO和KEGG富集分析以描述差异表达基因的生物学意义。最后，采用差异分析和免疫组织化学方法验证腺苷脱氨酶1 （ADAR1）的表达。结果：我们从Synapse数据库中评估了514例COAD患者的4079个RNA编辑位点。基于GNL3L、NUP43、MAGT1、EMP2和ARSD这5个与COAD患者预后显著相关的基因构建预后特征。单因素和多因素Cox回归分析显示，RNA编辑相关基因（RERGs）相关特征是COAD的独立危险因素。此外，实验证据表明ADAR1在结肠腺癌中高表达，沉默ADAR1可抑制癌细胞增殖。结论：基于5种RERGs建立了具有较强预测价值的预后模型。该模型不仅可以作为一种新的预后工具的基础，而且还有助于识别治疗COAD的潜在候选药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Medical Research 医学-医学：研究与实验

CiteScore

3.20

自引率

0.00%

发文量

247

审稿时长

>12 weeks

期刊介绍： European Journal of Medical Research publishes translational and clinical research of international interest across all medical disciplines, enabling clinicians and other researchers to learn about developments and innovations within these disciplines and across the boundaries between disciplines. The journal publishes high quality research and reviews and aims to ensure that the results of all well-conducted research are published, regardless of their outcome.