Characterization of Blue Light Receptors LreA and LreB in Aspergillus flavus.

Abstract

Light is a key external signal factor that regulates asexual development, stress resistance, and secondary metabolism in fungi. In the presence of light, photoreceptors sense several light receptors and affect fungal life. In this study, we characterized the function of the blue light receptors LreA and LreB in Aspergillus flavus, a potent pathogenic and toxigenic fungus. lreA or lreB deletion increased the growth rate but decreased conidial production in the presence or absence of light. The ΔlreA-mutant strain and the ΔlreB-mutant strain produced abnormal conidiophores, suggesting that lreA and lreB were essential for proper conidiation in A. flavus. The absence of lreA or lreB slightly decreased the stress response tolerance against thermal and oxidative stresses. In kernel infection, the ΔlreA mutant strain and the ΔlreB mutant strain produced conidia and aflatoxin B1 that were less than those produced by the control strains. Therefore, LreA and LreB play key roles in the growth, asexual development, and pathogenicity of A. flavus.