Biochemical characterization of antibodies submitted as reactive with glycophorin species

Abstract

Thirty-one antibodies submitted for testing were examined in order to determine with which glycophorin species they were reactive and what region of the target molecule(s) were involved in antigen recognition. Antibodies were assayed by direct and indirect aggmutination techniques, using cells of various MNSs phenotypes before and after exposure to various enzymes. In addition, antibodies were tested by immunoblotting and, in a small number of instances, by radioimmunoprecipitation, in order to determine or confirm the molecular species reactive with each antibody.