Antibodies associated with the Kell blood group system

Revue Fran?aise de Transfusion et Immuno-hématologie Pub Date : 1988-04-01 DOI:10.1016/S0338-4535(88)80126-0

W.L. Marsh, C.L. Johnson, B.I. Rabin

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Abstract

The Kell blood group presently includes 23 antigens [1], a null (K₀) phenotype, a number of phenotypes characterized by weak Kell antigen activity, and an independent antigen, designated Kx, which appears to play a role in Kell antigen expression.

Kell blood-group antigens are markers on the surface-exposed domain of a 93,000 daltons (93 kD) membrane glycoprotein [2]. Intrachain disulfide linkages [3], and probably the proper intra-membrane milieu, are important for the antigenic integrity of Kell 93 kD protein. Separated 93 kD Kell protein does not react by Western blot analysis with the Kell antibody used for its initial immuno-precipitation [1]. Incubation of intact red cells with solutions containing papain/DTT mixture [4] or 2-aminoethylisothiouronium bromide (AET) inactivates all antigens of the Kell complex except for Kx [5].

Five monoclonal antibodies have been examined for serological specificity within the Kell system and for their ability to recognize epitopes on a 93 kD red cell membrane protein by Western blot analysis.

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与凯尔血型系统相关的抗体

目前，Kell血型包括23种抗原[1]，一种null (K0)表型，一些以弱Kell抗原活性为特征的表型，以及一种被称为Kx的独立抗原，它似乎在Kell抗原表达中起作用。Kell血型抗原是位于93,000道尔顿(93 kD)膜糖蛋白[2]表面暴露区域的标记。链内二硫键[3]，可能还有适当的膜内环境，对Kell 93kd蛋白的抗原完整性很重要。Western blot分析分离的93 kD Kell蛋白与用于初始免疫沉淀[1]的Kell抗体不发生反应。用含有木瓜蛋白酶/DTT混合物[4]或2-氨基乙基异硫脲溴化铵(AET)的溶液孵育完整红细胞，使除Kx[5]外的所有Kell复合物抗原失活。通过Western blot分析，研究了5种单克隆抗体在Kell系统中的血清学特异性，以及它们识别93 kD红细胞膜蛋白表位的能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Revue Fran?aise de Transfusion et Immuno-hématologie

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