Serological and biochemical characterization of monoclonal antibodies against red cell markers related to expression of lutheran blood group antigens

Abstract

Monoclonal antibodies to erythrocyte membrane antigens have, prior to this investigation, led to identification of protein(s) which bear the Lu^b antigen [4] as well as to increased understanding of the effects of the in(Lu) gene [7, 8, 9, 10], a gene which acts in an autosomal dominant fashion to down-regulate expression of all Lutheran antigens as well as numerous other antigens recognized by human and murine monoclonal antisera [1, 2, 5]. This study was therefore designed to determine what, if any, further contribution could be made using the four submitted workshop antibodies toward the understanding of the biochemistry of Lutheran antigens and the mechanism of action of the In(Lu)