P. V. Konarev, L. N. Borschevskaya, T. L. Gordeeva, E. B. Pichkur, G. S. Peters, S. P. Sineokiy, V. R. Samygina
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引用次数: 0
Abstract
Samples of self-assembled particles based on the capsid protein of bacteriophage MS2 were obtained using different purification protocols and characterized. The study demonstrates the feasibility of using a combination of electron microscopy and small-angle X-ray scattering (SAXS) to select the optimal purification protocol. Electron microscopy allows the evaluation of self-assembly, while SAXS helps to estimate the statistical distribution of particles of different shapes and sizes in a sample. The best results were obtained using the PEG precipitation in combination with gel filtration and ultrafiltration.
研究人员采用不同的纯化方案获得了基于噬菌体 MS2 包囊蛋白的自组装颗粒样品，并对其进行了表征。该研究证明了结合使用电子显微镜和小角 X 射线散射（SAXS）来选择最佳纯化方案的可行性。电子显微镜可对自组装进行评估，而小角 X 射线散射则有助于估算样品中不同形状和大小颗粒的统计分布。将 PEG 沉淀与凝胶过滤和超滤结合使用可获得最佳结果。
期刊介绍：
Crystallography Reports is a journal that publishes original articles short communications, and reviews on various aspects of crystallography: diffraction and scattering of X-rays, electrons, and neutrons, determination of crystal structure of inorganic and organic substances, including proteins and other biological substances; UV-VIS and IR spectroscopy; growth, imperfect structure and physical properties of crystals; thin films, liquid crystals, nanomaterials, partially disordered systems, and the methods of studies.
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