Biological Evaluation of LL-37 Antimicrobial Peptide-Loaded Biodegradable Barrier Membranes in Guided Bone Regeneration

IF 3.9 3区化学 Q2 POLYMER SCIENCE Journal of Polymer Science Pub Date : 2024-12-11 DOI:10.1002/pol.20240781

Putida Pinthonglor, Thanapak Jaimalai, Donraporn Daranarong, Thannaphat Jenvoraphot, Punyaporn Pongsuwan, Nuttee Suree, Panchika Prangkio, Winita Punyodom, Nutchapon Chamusri, Burin Boonsri, Chayarop Supanchart

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Abstract

This study aims to determine the in vitro and in vivo biocompatibility and the differences in LL-37 releasing patterns of the PLC/Col/LL-37 sustained releasing membrane and the rapid releasing Col/LL-37 membrane for use as a barrier membrane in guided bone regeneration. On Days 3 and 7, the PLC/Col/LL-37 membrane revealed generated osteoclasts, whereas the PLC/Col membrane indicated mature osteoclasts. PLC/Col/LL-37 was non-toxic to blood cells and inhibited human albumin adhesion to the surface. A microbial reduction was observed in the Col/LL-37 membrane, while both PLC/Col/LL-37 and Col/LL-37 may have caused early phases of skin sensitization. In animal testing, PLC/Col/LL37 and Col/LL37 exhibited the same levels of inflammation as the control group at all time points, while the different releasing patterns of LL-37 loaded on PLC/Col/LL-37 and Col/LL-37 membranes had no effect on inflammation resolution in rat models.

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本研究旨在确定 PLC/Col/LL-37 持续释放膜和 Col/LL-37 快速释放膜在体外和体内的生物相容性以及 LL-37 释放模式的差异，以用作引导骨再生的屏障膜。在第 3 天和第 7 天，PLC/Col/LL-37 膜显示了生成的破骨细胞，而 PLC/Col 膜则显示了成熟的破骨细胞。PLC/Col/LL-37 对血细胞无毒，并能抑制人体白蛋白粘附到膜表面。在 Col/LL-37 膜上观察到微生物减少，而 PLC/Col/LL-37 和 Col/LL-37 都可能会引起皮肤过敏的早期阶段。在动物试验中，PLC/Col/LL-37 和 Col/LL37 在所有时间点表现出与对照组相同的炎症水平，而 PLC/Col/LL-37 和 Col/LL-37 膜上负载的 LL-37 的不同释放模式对大鼠模型的炎症缓解没有影响。

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来源期刊

Journal of Polymer Science POLYMER SCIENCE-

CiteScore

6.30

自引率

5.90%

发文量

264

期刊介绍： Journal of Polymer Research provides a forum for the prompt publication of articles concerning the fundamental and applied research of polymers. Its great feature lies in the diversity of content which it encompasses, drawing together results from all aspects of polymer science and technology. As polymer research is rapidly growing around the globe, the aim of this journal is to establish itself as a significant information tool not only for the international polymer researchers in academia but also for those working in industry. The scope of the journal covers a wide range of the highly interdisciplinary field of polymer science and technology.

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