Electrospun Nanofiber Membrane with Sustained Release of Mogroside V Enhances Alveolar Bone Defect Repair in Diabetic Rats.

Abstract

The impaired healing of alveolar bone defects in diabetic patients has attracted considerable attention, with Mogroside V (MV) emerging as a promising candidate due to its demonstrated antioxidation, hypoglycemic, and anti-inflammatory properties in patients with diabetes mellitus. To address the limitations of oral MV administration, such as low bioavailability, rapid metabolism, and a short half-life, we developed a nanofiber membrane utilizing electrospinning technology for topical application by preparing membranes using MV, chitosan (CS), nanohydroxyapatite (HA), and poly(vinyl alcohol) (PVA) as raw materials to prolong the effect of MV and enhance bone regeneration in diabetic patients. The MV/HA/PVA/CS exhibited a good fiber diameter, prolonged drug release, and suitable degradation time, along with other favorable properties. In vitro experiments revealed its excellent biocompatibility, effectiveness in promoting osteogenesis, upregulation of osteogenic and anti-inflammatory genes, and concurrent downregulation of pro-inflammatory genes. In vivo evaluations further confirmed its ability to effectively modulate the diabetic microenvironment, reduce bone damage, and facilitate anti-inflammatory effects and alveolar bone regeneration in diabetics. These findings suggest that a nanofiber membrane with sustained release of MV may serve as a promising biomaterial, providing new insights into improving the healing of diabetic alveolar bone defects.