Time-resolved X-ray solution scattering unveils the events leading to hemoglobin heme capture by staphylococcal IsdB

IF 15.7 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Nature Communications Pub Date : 2025-02-17 DOI:10.1038/s41467-024-54949-w
Omar De Bei, Marialaura Marchetti, Stefano Guglielmo, Eleonora Gianquinto, Francesca Spyrakis, Barbara Campanini, Stefano Bettati, Matteo Levantino, Luca Ronda
{"title":"Time-resolved X-ray solution scattering unveils the events leading to hemoglobin heme capture by staphylococcal IsdB","authors":"Omar De Bei, Marialaura Marchetti, Stefano Guglielmo, Eleonora Gianquinto, Francesca Spyrakis, Barbara Campanini, Stefano Bettati, Matteo Levantino, Luca Ronda","doi":"10.1038/s41467-024-54949-w","DOIUrl":null,"url":null,"abstract":"<p>Infections caused by <i>Staphylococcus aureus</i> depend on its ability to acquire nutrients. One essential nutrient is iron, which is obtained from the heme of the human host hemoglobin (Hb) through a protein machinery called Iron-regulated surface determinant (Isd) system. IsdB is the protein in charge of heme extraction from Hb, which is the first step of the chain of events leading to iron transfer to the bacterium cell interior. In order to elucidate the molecular events leading from the formation of the initial IsdB:Hb complex to heme extraction, we use time-resolved X-ray solution scattering (TR-XSS) in combination with rapid mixing triggering. We succeed in defining the stoichiometry of IsdB:Hb binding and in describing the kinetics of the subsequent structural changes. The presented approach is potentially applicable to unveil the complex kinetic pathways generated by protein-protein interaction in different biological systems.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"1 1","pages":""},"PeriodicalIF":15.7000,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature Communications","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.1038/s41467-024-54949-w","RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Infections caused by Staphylococcus aureus depend on its ability to acquire nutrients. One essential nutrient is iron, which is obtained from the heme of the human host hemoglobin (Hb) through a protein machinery called Iron-regulated surface determinant (Isd) system. IsdB is the protein in charge of heme extraction from Hb, which is the first step of the chain of events leading to iron transfer to the bacterium cell interior. In order to elucidate the molecular events leading from the formation of the initial IsdB:Hb complex to heme extraction, we use time-resolved X-ray solution scattering (TR-XSS) in combination with rapid mixing triggering. We succeed in defining the stoichiometry of IsdB:Hb binding and in describing the kinetics of the subsequent structural changes. The presented approach is potentially applicable to unveil the complex kinetic pathways generated by protein-protein interaction in different biological systems.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
时间分辨x射线溶液散射揭示导致葡萄球菌IsdB捕获血红蛋白血红素的事件
金黄色葡萄球菌引起的感染取决于它获取营养的能力。一种必需营养素是铁,它通过一种称为铁调节表面决定因子(Isd)系统的蛋白质机制从人宿主血红蛋白(Hb)的血红素中获得。IsdB是负责从Hb中提取血红素的蛋白质,这是导致铁转移到细菌细胞内部的一系列事件的第一步。为了阐明从初始IsdB:Hb复合物形成到血红素提取的分子事件,我们使用了时间分辨x射线溶液散射(TR-XSS)结合快速混合触发。我们成功地定义了IsdB:Hb结合的化学计量学,并描述了随后结构变化的动力学。提出的方法可能适用于揭示不同生物系统中蛋白质相互作用产生的复杂动力学途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Nature Communications
Nature Communications Biological Science Disciplines-
CiteScore
24.90
自引率
2.40%
发文量
6928
审稿时长
3.7 months
期刊介绍: Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.
期刊最新文献
Nucleotide-resolution mapping of regulatory elements via allelic readout of tiled base editing. Canonical BAF chromatin remodeling complex specifies stem cell fate via cell-type-specific co-factor recruitment. In-cell bypass diodes for high-efficiency and shading-tolerant back contact silicon photovoltaic modules. Lasing-like dynamics with virtual gain driven by complex-frequency excitations. Accurate prediction of tensorial spectra using equivariant graph neural network.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1