Analysis of interleukin-6 gene polymorphism and its serum levels in Indian age-related macular degeneration patients.

IF 1.4 3区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Vision Pub Date : 2024-12-28 eCollection Date: 2024-01-01

Anshu Yadav, Jitender Phogat, Manoj Yadav, Aarti Bhardwaj, Ritu Yadav, Manisha Nada, Manish Bhati, Supreme Goel, Rahul Thakur, Rakesh Kumar, Mayank Singh, Mukesh Tanwar

{"title":"Analysis of interleukin-6 gene polymorphism and its serum levels in Indian age-related macular degeneration patients.","authors":"Anshu Yadav, Jitender Phogat, Manoj Yadav, Aarti Bhardwaj, Ritu Yadav, Manisha Nada, Manish Bhati, Supreme Goel, Rahul Thakur, Rakesh Kumar, Mayank Singh, Mukesh Tanwar","doi":"","DOIUrl":null,"url":null,"abstract":"Background: Age-related macular degeneration (AMD) is a complex condition involving multiple factors. The condition is associated with numerous inflammatory indicators, including cytokines. Single-nucleotide polymorphisms in cytokine genes can also modify gene expression, perhaps contributing to the development of the disease. The objective of the present study was to examine the correlation among IL-6 SNPs (rs1800795, rs1800796, and rs1800797) and the serum levels of IL-6 in AMD patients treated at the Regional Institute of Ophthalmology of Pt. B.D. Sharma PGIMS, Rohtak (Haryana), India.Methods: This case-control study included 131 patients diagnosed with AMD using precise ophthalmic examinations, such as slit lamp examination, fundoscopy, and ocular coherence tomography. To provide a basis for comparison, we also enlisted 100 healthy individuals as controls. Serum IL-6 protein levels were measured in both patients and controls using an enzyme-linked immunosorbent assay kit (ELISA). Genotyping IL-6 SNPs was performed using the PCR and DNA Sanger sequencing technique.Results: IL-6 serum levels were considerably elevated in individuals with AMD compared to the control group (p < 0.05). Statistically significant differences were seen in the genotype frequencies of rs1800795 (p = 0.027) and rs1800797 (p = 0.0011) among the AMD patients and the healthy controls. Furthermore, strong correlations were observed between rs1800795 and the likelihood of developing AMD based on the heterozygous (OR = 2.04; p = 0.025), dominant (OR = 1.80; p = 0.035), and over-dominant models (OR = 2.10; p = 0.0094). Additionally, there were notable associations between rs1800797 and vulnerability to AMD through heterozygous (OR = 3.21; p = 0.009), dominant (OR = 2.74; p = 0.004), and over-dominant (OR = 3.11; p = 0.002) models. The rs1800795, rs1800796, and rs1800797 haplotypes C-G-A and G-G-A were linked to an elevated risk of AMD (p = 0.005, p = 0.024. respectively).Conclusions: Our findings indicated a significant elevation in IL-6 serum levels among the AMD patient group compared to the control group. The interleukin-6 gene polymorphisms rs1800795 and rs1800797 were linked to an elevated risk of AMD in our study population.","PeriodicalId":18866,"journal":{"name":"Molecular Vision","volume":"30 ","pages":"434-446"},"PeriodicalIF":1.4000,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829794/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Vision","FirstCategoryId":"3","ListUrlMain":"","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Age-related macular degeneration (AMD) is a complex condition involving multiple factors. The condition is associated with numerous inflammatory indicators, including cytokines. Single-nucleotide polymorphisms in cytokine genes can also modify gene expression, perhaps contributing to the development of the disease. The objective of the present study was to examine the correlation among IL-6 SNPs (rs1800795, rs1800796, and rs1800797) and the serum levels of IL-6 in AMD patients treated at the Regional Institute of Ophthalmology of Pt. B.D. Sharma PGIMS, Rohtak (Haryana), India.

Methods: This case-control study included 131 patients diagnosed with AMD using precise ophthalmic examinations, such as slit lamp examination, fundoscopy, and ocular coherence tomography. To provide a basis for comparison, we also enlisted 100 healthy individuals as controls. Serum IL-6 protein levels were measured in both patients and controls using an enzyme-linked immunosorbent assay kit (ELISA). Genotyping IL-6 SNPs was performed using the PCR and DNA Sanger sequencing technique.

Results: IL-6 serum levels were considerably elevated in individuals with AMD compared to the control group (p < 0.05). Statistically significant differences were seen in the genotype frequencies of rs1800795 (p = 0.027) and rs1800797 (p = 0.0011) among the AMD patients and the healthy controls. Furthermore, strong correlations were observed between rs1800795 and the likelihood of developing AMD based on the heterozygous (OR = 2.04; p = 0.025), dominant (OR = 1.80; p = 0.035), and over-dominant models (OR = 2.10; p = 0.0094). Additionally, there were notable associations between rs1800797 and vulnerability to AMD through heterozygous (OR = 3.21; p = 0.009), dominant (OR = 2.74; p = 0.004), and over-dominant (OR = 3.11; p = 0.002) models. The rs1800795, rs1800796, and rs1800797 haplotypes C-G-A and G-G-A were linked to an elevated risk of AMD (p = 0.005, p = 0.024. respectively).

Conclusions: Our findings indicated a significant elevation in IL-6 serum levels among the AMD patient group compared to the control group. The interleukin-6 gene polymorphisms rs1800795 and rs1800797 were linked to an elevated risk of AMD in our study population.

Abstract Image

微信好友朋友圈 QQ好友复制链接

本刊更多论文

印度老年性黄斑变性患者白细胞介素-6基因多态性及其血清水平分析。

背景：老年性黄斑变性（AMD）是一种涉及多种因素的复杂疾病。这种情况与许多炎症指标有关，包括细胞因子。细胞因子基因的单核苷酸多态性也可以改变基因表达，可能有助于疾病的发展。本研究的目的是检查IL-6 snp （rs1800795、rs1800796和rs1800797）与在印度罗塔克（哈里亚纳邦）地区眼科研究所接受治疗的AMD患者血清IL-6水平的相关性。方法：本病例对照研究纳入131例经裂隙灯检查、眼底镜检查、眼相干断层扫描等精确眼科检查诊断为AMD的患者。为了提供比较的基础，我们还招募了100名健康个体作为对照。采用酶联免疫吸附测定试剂盒（ELISA）测定患者和对照组血清IL-6蛋白水平。采用PCR和DNA Sanger测序技术对IL-6 snp进行基因分型。结果：与对照组相比，AMD患者血清IL-6水平明显升高（p < 0.05）。AMD患者与健康对照组rs1800795、rs1800797基因型频率差异有统计学意义（p = 0.027）。此外，rs1800795与基于杂合子的AMD发生可能性之间存在很强的相关性(OR = 2.04；p = 0.025)，显性(OR = 1.80；p = 0.035)和过优势模型(OR = 2.10；P = 0.0094)。此外，rs1800797与AMD易感性通过杂合存在显著相关性(OR = 3.21；p = 0.009)，显性(OR = 2.74；p = 0.004)和过显性(OR = 3.11；P = 0.002)模型。rs1800795、rs1800796和rs1800797单倍型C-G-A和G-G-A与AMD的风险升高有关（p = 0.005, p = 0.024）。分别)。结论：我们的研究结果表明，与对照组相比，AMD患者组血清IL-6水平显著升高。在我们的研究人群中，白介素-6基因多态性rs1800795和rs1800797与AMD的风险升高有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Molecular Vision 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Molecular Vision is a peer-reviewed journal dedicated to the dissemination of research results in molecular biology, cell biology, and the genetics of the visual system (ocular and cortical). Molecular Vision publishes articles presenting original research that has not previously been published and comprehensive articles reviewing the current status of a particular field or topic. Submissions to Molecular Vision are subjected to rigorous peer review. Molecular Vision does NOT publish preprints. For authors, Molecular Vision provides a rapid means of communicating important results. Access to Molecular Vision is free and unrestricted, allowing the widest possible audience for your article. Digital publishing allows you to use color images freely (and without fees). Additionally, you may publish animations, sounds, or other supplementary information that clarifies or supports your article. Each of the authors of an article may also list an electronic mail address (which will be updated upon request) to give interested readers easy access to authors.