An axolotl limb regeneration-inspired strategy to enhance alveolar bone regeneration

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL Bioactive Materials Pub Date : 2025-06-01 Epub Date: 2025-02-19 DOI:10.1016/j.bioactmat.2025.02.020

Rongpu Liu , Guifang Wang , Li Ma , Guangzheng Yang , Sihan Lin , Ningjia Sun , Jiajia Wang , Huijing Ma , Xinquan Jiang , Wenjie Zhang

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Abstract

Guided bone regeneration (GBR) is widely applied in implant dentistry, employing barrier membranes to create an osteogenic space by preventing gingival tissue ingrowth. However, this method does not enhance the osteogenic capacity of osteoblasts, limiting sufficient bone volume in larger defects. Inspired by axolotl limb regeneration, abundant soft tissue-derived stem cells mobilized to the defect may facilitate comprehensive osteogenesis within a BMP-2-enriched environment. We developed a biomimetic channel system (BCS) to promote alveolar bone regeneration, using channel structures to activate gingival-derived stem cells under a BMP-2-enriched biological barrier. In a cell-tracing mouse model, Prrx1⁺ stem cells demonstrated a critical role in BMP-2-induced subcutaneous osteogenesis. Sequencing and histological analyses revealed that channel structures significantly enhance soft tissue cell proliferation and migration. Attributable to the biological barrier, BCS applications markedly improved bone formation in beagle mandibular defects. These results suggest a novel osteoinductive strategy for alveolar bone regeneration that functions without a traditional barrier membrane.

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一种以蝾螈肢体再生为灵感的策略来促进牙槽骨再生

引导骨再生（Guided bone regeneration， GBR）是一种利用屏障膜来防止牙龈组织向内生长从而形成成骨空间的方法，在种植牙医学中得到了广泛的应用。然而，这种方法不能提高成骨细胞的成骨能力，限制了较大缺陷的足够骨容量。受蝾螈肢体再生的启发，大量的软组织来源的干细胞被动员到缺陷中，可能在富含bmp -2的环境中促进全面的成骨。我们开发了一种仿生通道系统（BCS）来促进牙槽骨再生，利用通道结构在富含bmp -2的生物屏障下激活牙龈来源的干细胞。在细胞追踪小鼠模型中，Prrx1+干细胞在bmp -2诱导的皮下成骨过程中发挥了关键作用。测序和组织学分析显示，通道结构显著促进软组织细胞的增殖和迁移。由于生物屏障的作用，BCS应用显著改善了小猎犬下颌缺损的骨形成。这些结果提示了一种新的骨诱导策略，可以在没有传统屏障膜的情况下进行牙槽骨再生。

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产品信息

阿拉丁

gelatin

来源期刊

Bioactive Materials Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

28.00

自引率

6.30%

发文量

436

审稿时长

20 days

期刊介绍： Bioactive Materials is a peer-reviewed research publication that focuses on advancements in bioactive materials. The journal accepts research papers, reviews, and rapid communications in the field of next-generation biomaterials that interact with cells, tissues, and organs in various living organisms. The primary goal of Bioactive Materials is to promote the science and engineering of biomaterials that exhibit adaptiveness to the biological environment. These materials are specifically designed to stimulate or direct appropriate cell and tissue responses or regulate interactions with microorganisms. The journal covers a wide range of bioactive materials, including those that are engineered or designed in terms of their physical form (e.g. particulate, fiber), topology (e.g. porosity, surface roughness), or dimensions (ranging from macro to nano-scales). Contributions are sought from the following categories of bioactive materials: Bioactive metals and alloys Bioactive inorganics: ceramics, glasses, and carbon-based materials Bioactive polymers and gels Bioactive materials derived from natural sources Bioactive composites These materials find applications in human and veterinary medicine, such as implants, tissue engineering scaffolds, cell/drug/gene carriers, as well as imaging and sensing devices.