{"title":"On possibility of fluorescence detection of dabsylated amino acids in liquid chromatography","authors":"Dariya I. Ziganshina, Leonid D. Asnin","doi":"10.1016/j.chroma.2025.465777","DOIUrl":null,"url":null,"abstract":"<div><div>Dabsyl chloride is a derivatization reagent used for UV/Vis detection of amino compounds. Since the dabsyl group does not fluoresce, it has never been monitored with a fluorescent (FL) detector. The present report shows that dabsylated amino acids exemplified by serine enantiomers produce appreciable negative peaks with an FL detector due to a strong quenching effect of the dabsyl group; thus, their fluorescent determination is possible. This finding extends a repertoire of derivatizing reagents for chromatographs without a UV/Vis detector (but with an FL one). Calibration dependencies for L- and D-Ser were similar with UV/Vis and FL detectors, although the limit of detection for the former (8 μg (L), 20 μg (D)) was lower than for the latter (48 μg (L), 42 μg (D)) due to larger noise of the FL detector.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1746 ","pages":"Article 465777"},"PeriodicalIF":3.8000,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chromatography A","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0021967325001256","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Dabsyl chloride is a derivatization reagent used for UV/Vis detection of amino compounds. Since the dabsyl group does not fluoresce, it has never been monitored with a fluorescent (FL) detector. The present report shows that dabsylated amino acids exemplified by serine enantiomers produce appreciable negative peaks with an FL detector due to a strong quenching effect of the dabsyl group; thus, their fluorescent determination is possible. This finding extends a repertoire of derivatizing reagents for chromatographs without a UV/Vis detector (but with an FL one). Calibration dependencies for L- and D-Ser were similar with UV/Vis and FL detectors, although the limit of detection for the former (8 μg (L), 20 μg (D)) was lower than for the latter (48 μg (L), 42 μg (D)) due to larger noise of the FL detector.
期刊介绍：
The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.
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