Pattern-based rapid and robust profiling of gut microbiota and its metabolism with a single fluorescent probe

IF 9.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Science China Chemistry Pub Date : 2024-10-14 DOI:10.1007/s11426-024-2191-y

Hongli Tan, Meizi Zeng, Cong Fang, Xiaohua Zhu, Feng Liu, Ying Long, Ruijie Deng, Peng Yin, Meiling Liu, Haitao Li, Youyu Zhang, Shouzhuo Yao

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Abstract

The gut microbiota and the associated metabolism play a pivotal role in maintaining human health, yet their assessment is challenging due to their inherent diversity, variety, and complexity. Herein, we developed a multichannel sensor array based on a single fluorescent probe, allowing for rapid and robust profiling gut microbiota and their key metabolites including cysteine (Cys), glutathione (GSH), and homocysteine (Hcy). The assay leverages the single fluorescent probe that is with multiple binding sites and the cross-reactive sensing principle to specifically recognize different biological thiols. By analyzing the pattern of biological thiols, the assay is capable of rapidly identifying six gut-derived bacteria including probiotics, neutral, and pathogenic strains based on fluorescent fingerprints within 5 min, and also discriminating bacteria and their mixtures with different composition. Using the assay that enables the simultaneous measurement of multiple gut-derived bacteria and their metabolites, the designed array achieved an accuracy of 0.99 when discriminating colorectal cancer (CRC) patient feces samples from healthy individuals. Remarkably, the as-prepared sensor array can also be used to identify various stages of CRC. The simplicity, rapidness, and cost-effectiveness of our approach render it a robust platform for the analysis of gut microbiota.

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基于模式的快速和强大的肠道微生物群及其代谢分析与单一荧光探针

肠道菌群及其相关代谢在维持人体健康中起着关键作用，但由于其固有的多样性、多样性和复杂性，对其进行评估具有挑战性。在此，我们开发了一种基于单一荧光探针的多通道传感器阵列，允许快速和稳健地分析肠道微生物群及其关键代谢物，包括半胱氨酸（Cys），谷胱甘肽（GSH）和同型半胱氨酸（Hcy）。该检测利用具有多个结合位点的单荧光探针和交叉反应传感原理特异性识别不同的生物硫醇。通过分析生物硫醇的分布规律，该方法可在5 min内根据荧光指纹快速鉴定出益生菌、中性菌和致病菌等6种肠道源性细菌，并可区分不同组成的细菌及其混合物。使用该检测方法，可以同时测量多种肠道来源的细菌及其代谢物，设计的阵列在区分结直肠癌（CRC）患者粪便样本和健康个体时达到了0.99的准确性。值得注意的是，制备的传感器阵列也可用于识别CRC的各个阶段。该方法的简单、快速和成本效益使其成为分析肠道微生物群的强大平台。

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来源期刊

Science China Chemistry CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

14.40

自引率

7.30%

发文量

3787

审稿时长

2.2 months

期刊介绍： Science China Chemistry, co-sponsored by the Chinese Academy of Sciences and the National Natural Science Foundation of China and published by Science China Press, publishes high-quality original research in both basic and applied chemistry. Indexed by Science Citation Index, it is a premier academic journal in the field. Categories of articles include: Highlights. Brief summaries and scholarly comments on recent research achievements in any field of chemistry. Perspectives. Concise reports on thelatest chemistry trends of interest to scientists worldwide, including discussions of research breakthroughs and interpretations of important science and funding policies. Reviews. In-depth summaries of representative results and achievements of the past 5–10 years in selected topics based on or closely related to the research expertise of the authors, providing a thorough assessment of the significance, current status, and future research directions of the field.