Association of SIRT1 rs3758391 Polymorphism With T2DM in Bangladeshi Population: Evidence From a Case-Control Study and Meta-Analysis

Abstract

Background and Aim

Type 2 diabetes mellitus (T2DM) remains one of the major causes of morbidity and mortality worldwide, including Bangladesh. SIRT1, an NAD-dependent deacetylase, is involved in energy homeostasis and protects β-cells of the pancreas from oxidative stress. Single nucleotide polymorphisms (SNPs) in the SIRT1 gene have been found to be associated with T2DM in several populations, however, with conflicting results. The aim of this present case-control study, along with the meta-analysis, was to elucidate the association of rs3758391 polymorphism with the susceptibility to T2DM in Bangladeshi population.

Methods

72 T2DM patients and 90 healthy controls were enrolled in our study and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed for genotyping the SNP. Odds ratio (OR) with 95% confidence interval (95% CI) was used to represent the association of SIRT1 polymorphism with T2DM. For the meta-analysis six studies were included and pooled odds ratio with 95% CI were calculated for six genetic models using the random effects model. Heterogeneity and publication bias was also calculated for each study.

Results

A significant association was found between rs3758391 polymorphism and increased risk of T2DM under codominant TT versus CC (OR = 3.88, 95% CI = 1.34–11.25, p = 0.012), recessive TT versus CC + CT (OR = 2.83, 95% CI = 1.12–7.09, p = 0.027) and allelic T versus C (OR = 1.67, 95% CI = 1.07–2.60, p = 0.024) genetic models. However, no significant association between rs3758391 and other biochemical and anthropometric parameters were found. Our meta-analysis showed no statistically significant association of this polymorphism.

Conclusion

We conclude that, polymorphism at rs3758391 of SIRT1 gene conferred an increased risk of T2DM in Bangladeshi population.