Molecular characterization, chemical profile and biological properties of essential oils from Chamaemelum nobile (L.) flowers of Morocco: in vitro and in silico studies.
El-Mehdi El-Assri, Youssef El-Assri, Rajae El Brahimi, Mohamed El Fadili, Asmae Baghouz, Hatem A Abuelizz, Sara Er-Rahmani, Amal Lahkimi, Abdelhak Bouia
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引用次数: 0
Abstract
Introduction: This study investigated the antioxidant, antimicrobial, and insecticidal properties of Chamaemelum nobile (L.) essential oil (CN-EO), harvested in Taounate, Morocco. The molecular composition and chemical profile of CN-EO were also characterized.
Methods: The CN-EO was extracted using a Clevenger apparatus. Its chemical composition was analyzed using gas chromatography-mass spectrometry (GC-MS). Antioxidant activity was evaluated using the DPPH assay, while antimicrobial properties were assessed via the disk diffusion method to measure inhibition zones against various bacterial and fungal strains. Insecticidal activity was tested through bioassays to determine insect mortality and repellency rates. Phylogenetic analysis of DNA sequences was conducted to confirm the species identity.
Results: GC-MS analysis identified 24 compounds in CN-EO, with β-Oplopenone (18.66%), Spathulenol (14.90%), and Himachalene (12.47%) as major constituents. CN-EO exhibited strong antioxidant activity (IC50 = 135.8 ± 1.03 μg/mL). Antimicrobial assays revealed inhibition zones of up to 20.67 ± 0.58 mm (Staphylococcus aureus) and antifungal inhibition of 40.42% ± 2.82% against Aspergillus flavus. Insecticidal tests showed total insect mortality at 166 µL/L within 48 h and a 60% repellent effect. Phylogenetic analysis of the DNA sequence revealed a 99.22% similarity with Chamaemelum nobile (L.).
Conclusion: These results demonstrate the significant potential of Moroccan CN-EO in phytomedicine. It exhibits a wide range of biological activities and shows great promise as a natural antioxidant, antimicrobial agent, antifungal, and insecticide.
期刊介绍：
Frontiers in Chemistry is a high visiblity and quality journal, publishing rigorously peer-reviewed research across the chemical sciences. Field Chief Editor Steve Suib at the University of Connecticut is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to academics, industry leaders and the public worldwide.
Chemistry is a branch of science that is linked to all other main fields of research. The omnipresence of Chemistry is apparent in our everyday lives from the electronic devices that we all use to communicate, to foods we eat, to our health and well-being, to the different forms of energy that we use. While there are many subtopics and specialties of Chemistry, the fundamental link in all these areas is how atoms, ions, and molecules come together and come apart in what some have come to call the “dance of life”.
All specialty sections of Frontiers in Chemistry are open-access with the goal of publishing outstanding research publications, review articles, commentaries, and ideas about various aspects of Chemistry. The past forms of publication often have specific subdisciplines, most commonly of analytical, inorganic, organic and physical chemistries, but these days those lines and boxes are quite blurry and the silos of those disciplines appear to be eroding. Chemistry is important to both fundamental and applied areas of research and manufacturing, and indeed the outlines of academic versus industrial research are also often artificial. Collaborative research across all specialty areas of Chemistry is highly encouraged and supported as we move forward. These are exciting times and the field of Chemistry is an important and significant contributor to our collective knowledge.
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