Long non-coding RNA AK007111 mediates mast cells apoptosis via targeting of protein MOAP1.

IF 1.3 4区医学 Q3 ALLERGY Journal of Asthma Pub Date : 2025-07-01 Epub Date: 2025-02-20 DOI:10.1080/02770903.2025.2463974

Xiao Ma, Yingying Luo, Jiejing Xu, Feng Liu, Changdi Xu, Heng Tang

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Abstract

Background: Long non-coding RNAs (lncRNAs) are emerging regulators of pathophysiological processes in a variety of diseases including asthma. In this study, we reported the identification of lncRNA-AK007111 as an essential modulator of mast cell apoptosis and investigated its potential mechanism.

Methods: RNA-seq profiling and transcriptome sequencing technology were adopted to screen for differentially expressed genes. Transfection was done by small interfering RNAs (siRNAs) to down-regulate lncRNA-AK007111 and Modulator of apoptosis 1 (MOAP1). Starvation was used to induce apoptosis. The apoptotic rate was measured by flow cytometry. Western Blot was conducted to detect the expression of apoptosis-related proteins.

Results: LncRNA-AK007111 was highly expressed in IgE/Ag-mediated activation of mast cells. Down-regulation of LncRNA-AK007111 promoted apoptosis of mast cells. Down-regulation of MOAP1 attenuated apoptosis in mast cells induced by the down-regulation of lncRNA-AK007111.

Conclusion: LncRNA-AK007111 may be a potential regulator of mast cell apoptosis by interaction with MOAP1.

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长链非编码RNA AK007111通过靶向蛋白MOAP-1介导肥大细胞凋亡

背景：长链非编码rna （lncRNAs）是包括哮喘在内的多种疾病病理生理过程的新兴调控因子。在本研究中，我们报道了lncRNA-AK007111作为肥大细胞凋亡的必要调节剂的鉴定，并探讨了其潜在的机制。方法：采用RNA-seq分析和转录组测序技术筛选差异表达基因。转染小干扰rna （sirna）下调lncRNA-AK007111和凋亡调节剂1 （Moap1）。饥饿诱导细胞凋亡。流式细胞术检测细胞凋亡率，western Blot检测细胞凋亡相关蛋白的表达。结果：LncRNA-AK007111在IgE/ ag介导的肥大细胞活化中高表达。LncRNA-AK007111的下调促进肥大细胞凋亡。下调MOAP1可减弱lncRNA-AK007111下调诱导的肥大细胞凋亡。结论：LncRNA-AK007111可能通过与MOAP-1相互作用而成为肥大细胞凋亡的潜在调节因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Asthma 医学-过敏

CiteScore

4.00

自引率

5.30%

发文量

158

审稿时长

3-8 weeks

期刊介绍： Providing an authoritative open forum on asthma and related conditions, Journal of Asthma publishes clinical research around such topics as asthma management, critical and long-term care, preventative measures, environmental counselling, and patient education.