Her-2 nanobody modified cisplatin nanoparticles for precise chemotherapy of colon cancer.

IF 3.2 4区医学 Q2 CHEMISTRY, APPLIED Journal of microencapsulation Pub Date : 2025-05-01 Epub Date: 2025-02-20 DOI:10.1080/02652048.2025.2467060

Weina Liang, Yan Zhang, Jianpeng Li, Chenglin Ji, Xuexin Jiang

{"title":"Her-2 nanobody modified cisplatin nanoparticles for precise chemotherapy of colon cancer.","authors":"Weina Liang, Yan Zhang, Jianpeng Li, Chenglin Ji, Xuexin Jiang","doi":"10.1080/02652048.2025.2467060","DOIUrl":null,"url":null,"abstract":"Construct a Her-2 nanobody modified nanoplatform as a versatile carrier of cisplatin and evaluate its anti-tumour effects. Size, morphology, cellular uptake, in vitro release, cell viability, bio-distribution and antitumor efficacy were respectively measured by dynamic light scattering, transmission electron microscopy, confocal microscopy, HPLC, MTT assay, ICP-Mass and tumour volume. Nb-CDDP NPs was prepared with average diameter 60.4 ± 8.4 nm, PDI 0.2 ± 0.02, Zeta potential -35.74 mV, entrapment efficiency 89.5%±0.8% and drug loading 28.7%±1.3% (w/w). From which cisplatin could release more rapidly in acidic solution. NPs could be easily phagocytised and exhibited stronger cytotoxic effect in HCT-116 cells with IC50 1.46 ± 0.019 μg/mL. The concentration of Nb-CDDP NPs in tumour and its inhibition ratio on tumour volume were both higher than without Nb modification, with hardly any influence on body weight. This cisplatin nanoplatform exhibits exceptional properties and high targeting anti-tumour efficacy in colon cancer cells and mice, which maybe provide a promising strategy for precise chemotherapy.","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":" ","pages":"230-238"},"PeriodicalIF":3.2000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microencapsulation","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/02652048.2025.2467060","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/20 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 0

Abstract

Construct a Her-2 nanobody modified nanoplatform as a versatile carrier of cisplatin and evaluate its anti-tumour effects. Size, morphology, cellular uptake, in vitro release, cell viability, bio-distribution and antitumor efficacy were respectively measured by dynamic light scattering, transmission electron microscopy, confocal microscopy, HPLC, MTT assay, ICP-Mass and tumour volume. Nb-CDDP NPs was prepared with average diameter 60.4 ± 8.4 nm, PDI 0.2 ± 0.02, Zeta potential -35.74 mV, entrapment efficiency 89.5%±0.8% and drug loading 28.7%±1.3% (w/w). From which cisplatin could release more rapidly in acidic solution. NPs could be easily phagocytised and exhibited stronger cytotoxic effect in HCT-116 cells with IC₅₀ 1.46 ± 0.019 μg/mL. The concentration of Nb-CDDP NPs in tumour and its inhibition ratio on tumour volume were both higher than without Nb modification, with hardly any influence on body weight. This cisplatin nanoplatform exhibits exceptional properties and high targeting anti-tumour efficacy in colon cancer cells and mice, which maybe provide a promising strategy for precise chemotherapy.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Her-2纳米体修饰的顺铂纳米颗粒用于结肠癌的精确化疗。

构建Her-2纳米体修饰的纳米平台作为顺铂的多功能载体，并评价其抗肿瘤作用。采用动态光散射、透射电镜、共聚焦显微镜、高效液相色谱、MTT法、ICP-Mass法和肿瘤体积法分别测定其大小、形态、细胞摄取、体外释放、细胞活力、生物分布和抗肿瘤疗效。制备的Nb-CDDP NPs平均直径60.4±8.4 nm, PDI 0.2±0.02，Zeta电位-35.74 mV，包封效率89.5%±0.8%，载药量28.7%±1.3% （w/w）。顺铂在酸性溶液中释放速度更快。NPs对HCT-116细胞具有较强的细胞毒作用，IC50为1.46±0.019 μg/mL。Nb- cddp NPs在肿瘤中的浓度及其对肿瘤体积的抑制率均高于未修饰Nb的肿瘤，对体重几乎没有影响。该顺铂纳米平台在结肠癌细胞和小鼠中表现出独特的特性和高靶向抗肿瘤效果，可能为精确化疗提供一种有前景的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.