[GRIP1 is Involved in the Interaction of Vimentin Filaments with Focal Adhesions in Endothelial Cells].

Abstract

Vimentin intermediate filaments are dynamic structures that are able to move in cytoplasm owing to activity of the motor proteins, kinesin-1 and cytoplasmic dynein. How exactly motors interact with vimentin filaments remains unclear. In this work, I show that GRIP1 (Glutamate Receptor Interacting Protein 1), known as adapter for kinesin-1 on many cargoes in neurons, might also mediate kinesin-1 interaction with vimentin filaments. GRIP1 associates with vimentin filaments in various cells and co-immunoprecipitates with vimentin from cell lysates. Human endothelial cells knockout by GRIP1 gene lose focal adhesions and change their adhesive properties. Hypothetically, kinesin-1 engages GRIP1 to deliver vimentin filaments to the cell periphery so that they make contact with focal adhesions and stabilize them.