Ultrasonic Vocalization Acoustics After Recurrent Laryngeal Nerve Injury and Recovery

IF 2 3区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL Laryngoscope Pub Date : 2025-02-20 DOI:10.1002/lary.32069

Robert Morrison, Arlin Khan, Adrianna C. Shembel

{"title":"Ultrasonic Vocalization Acoustics After Recurrent Laryngeal Nerve Injury and Recovery","authors":"Robert Morrison, Arlin Khan, Adrianna C. Shembel","doi":"10.1002/lary.32069","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objectives</h3>\n \n <p>The lack of well-defined functional acoustic correlates of vocal fold paralysis in animal models creates challenges for translating basic science studies of recurrent laryngeal nerve (RLN) injury to patients with vocal fold paralysis. Capitalizing on natural communicative rodent ultrasonic vocalizations (USVs), this study aimed to (1) investigate the impact of RLN injury on USV acoustics in both acute and chronic stages of RLN injury and (2) to identify USV parameters with the greatest ecological validity specific to RLN injury.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>USVs were recorded over 2 weeks (acute) in 16 animals and over 30 weeks (chronic) in 8 animals using two USV elicitation paradigms (<i>n</i> = 16 non-elicited, spontaneous; <i>n</i> = 8 elicited, sexually paired). USV acoustic characteristics from a list of possible parameters (duration, principal frequency, complexity, power, and tonality) were compared across USV elicitation paradigms and time points (before RLN injury, 2 weeks after injury, and 30 weeks after injury).</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The USV parameters most impacted by RLN injury were frequency, power, and tonality, with significant decreases in all three parameters in both non-elicited/spontaneous and sexually elicited USV elicitation paradigms. All USV parameters were stable across 30 weeks after RLN injury.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>USV acoustic frequency, power, and tonality best characterize RLN injury and are stable over time, demonstrating their ecological utility in characterizing RLN injury and recovery. Furthermore, their stability can ensure accurate outcome metrics for future studies. Finally, these parameters have direct translation to humans with vocal fold paralysis.</p>\n </section>\n \n <section>\n \n <h3> Level of Evidence</h3>\n \n <p>NA</p>\n </section>\n </div>","PeriodicalId":49921,"journal":{"name":"Laryngoscope","volume":"135 7","pages":"2445-2453"},"PeriodicalIF":2.0000,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lary.32069","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Laryngoscope","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/lary.32069","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives

The lack of well-defined functional acoustic correlates of vocal fold paralysis in animal models creates challenges for translating basic science studies of recurrent laryngeal nerve (RLN) injury to patients with vocal fold paralysis. Capitalizing on natural communicative rodent ultrasonic vocalizations (USVs), this study aimed to (1) investigate the impact of RLN injury on USV acoustics in both acute and chronic stages of RLN injury and (2) to identify USV parameters with the greatest ecological validity specific to RLN injury.

Methods

USVs were recorded over 2 weeks (acute) in 16 animals and over 30 weeks (chronic) in 8 animals using two USV elicitation paradigms (n = 16 non-elicited, spontaneous; n = 8 elicited, sexually paired). USV acoustic characteristics from a list of possible parameters (duration, principal frequency, complexity, power, and tonality) were compared across USV elicitation paradigms and time points (before RLN injury, 2 weeks after injury, and 30 weeks after injury).

Results

The USV parameters most impacted by RLN injury were frequency, power, and tonality, with significant decreases in all three parameters in both non-elicited/spontaneous and sexually elicited USV elicitation paradigms. All USV parameters were stable across 30 weeks after RLN injury.

Conclusion

USV acoustic frequency, power, and tonality best characterize RLN injury and are stable over time, demonstrating their ecological utility in characterizing RLN injury and recovery. Furthermore, their stability can ensure accurate outcome metrics for future studies. Finally, these parameters have direct translation to humans with vocal fold paralysis.

Level of Evidence

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

喉返神经损伤及恢复后的超声发声声学。

目的：在动物模型中缺乏明确定义的声带麻痹功能声学相关性，为将喉返神经（RLN）损伤的基础科学研究转化为声带麻痹患者带来了挑战。利用啮齿类动物的自然交流超声发声（USV），本研究旨在(1)研究RLN损伤在急性和慢性阶段对USV声学的影响；(2)确定RLN损伤特有的最具生态效度的USV参数。方法：采用两种USV激发模式(n = 16例非激发、自发；N = 8引出，性配对)。通过一系列可能的参数（持续时间、主频率、复杂性、功率和音调）来比较不同USV引出模式和时间点（RLN损伤前、损伤后2周和损伤后30周）的USV声学特征。结果：RLN损伤对USV参数影响最大的是频率、功率和调性，在非诱发/自发和性诱发USV诱发范式中，这三个参数均显著降低。所有USV参数在RLN损伤后30周内保持稳定。结论：USV的频率、功率和音调最能表征RLN损伤，且随时间稳定，证明了它们在表征RLN损伤和恢复方面的生态效用。此外，它们的稳定性可以确保未来研究的准确结果指标。最后，这些参数对声带麻痹患者有直接的翻译作用。证据级别：无。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Laryngoscope 医学-耳鼻喉科学

CiteScore

6.50

自引率

7.70%

发文量

500

审稿时长

2-4 weeks

期刊介绍： The Laryngoscope has been the leading source of information on advances in the diagnosis and treatment of head and neck disorders since 1890. The Laryngoscope is the first choice among otolaryngologists for publication of their important findings and techniques. Each monthly issue of The Laryngoscope features peer-reviewed medical, clinical, and research contributions in general otolaryngology, allergy/rhinology, otology/neurotology, laryngology/bronchoesophagology, head and neck surgery, sleep medicine, pediatric otolaryngology, facial plastics and reconstructive surgery, oncology, and communicative disorders. Contributions include papers and posters presented at the Annual and Section Meetings of the Triological Society, as well as independent papers, "How I Do It", "Triological Best Practice" articles, and contemporary reviews. Theses authored by the Triological Society’s new Fellows as well as papers presented at meetings of the American Laryngological Association are published in The Laryngoscope. • Broncho-esophagology • Communicative disorders • Head and neck surgery • Plastic and reconstructive facial surgery • Oncology • Speech and hearing defects