A novel solution for real-time in-situ cell distribution monitoring in 3D bioprinting via fluorescence imaging.

IF 8.2 2区 医学 Q1 ENGINEERING, BIOMEDICAL Biofabrication Pub Date : 2025-02-20 DOI:10.1088/1758-5090/adb891
Alessandro Margarita, Simone Giovanni Gugliandolo, Silvia Santoni, Davide Moscatelli, Bianca Maria Colosimo
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引用次数: 0

Abstract

3D bioprinting is rapidly evolving as a transformative technology for constructing biological tissues with precise cell and bioink placement. However, ensuring the quality and viability of bioprinted structures presents significant challenges, highlighting the need for advanced monitoring systems. Our study introduces a space-efficient, non-invasive approach for real-time, in-situ monitoring of cell dispersion in bioprinted constructs. Utilizing a novel in-situ fluorescence microscopy technique, we employ nanoparticles for cell tagging and integrate a compact digital microscope into the bioprinter for layer-by-layer imaging, significantly saving space and weight to make the solution adaptable to any commercial bioprinter. This method enhances in-situ analysis by combining data from the fluorescence system with conventional visible spectrum imaging. The synergy of these datasets provides a detailed method to examine cell dispersion and facilitates continuous monitoring during the bioprinting process. This allows for the immediate identification and correction of irregularities in cell deposition. Our approach aims to advance 3D bioprinting, setting new standards for the reliability and efficiency of bioprinted structures.

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来源期刊
Biofabrication
Biofabrication ENGINEERING, BIOMEDICAL-MATERIALS SCIENCE, BIOMATERIALS
CiteScore
17.40
自引率
3.30%
发文量
118
审稿时长
2 months
期刊介绍: Biofabrication is dedicated to advancing cutting-edge research on the utilization of cells, proteins, biological materials, and biomaterials as fundamental components for the construction of biological systems and/or therapeutic products. Additionally, it proudly serves as the official journal of the International Society for Biofabrication (ISBF).
期刊最新文献
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