Assessment of the Cytotoxicity Mechanism of Diazinon on HFFF2 Cells: A Bioinformatic and Experimental Study

IF 3.2 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Biochemical and Molecular Toxicology Pub Date : 2025-02-23 DOI:10.1002/jbt.70146

Mehdi Sarailoo, Vahid Asghariazar, Sina Seifimansour, Mahtab Kadkhodayi, Erfan Zare, Parnia Vajdi, Mehdi Asghari Vostakolaei

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Abstract

Pesticide exposure can cause many skin diseases such as hypopigmentation and contact dermatitis, but the underlying mechanisms remain unclear. Furthermore, Organophosphate pesticides (OPs) including Diazinon (DZN) can affect cellular pathways like ATPase, leading to mitochondrial energy deficit and even apoptosis in the cell's functions. Following cell exposure to the OP pesticide DZN through treatment, we evaluated alteration in gene expression and DNA damage. Bioinformatic analysis was performed based on the AutoDock, Protein Data Bank, STRING, Way2Drug, and Comparative Toxicogenomics databases and tools. The MTT assay, wound healing, DAPI staining, flow cytometry, and real-time PCR were applied in the current study. The results showed that the viability and migration capacity of HFFF2 cells decreased, and the apoptosis rate increased in the DZN-treated group. These findings revealed that DZN regulated the expression of the apoptotic genes in DZN cells.

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接触杀虫剂可导致色素沉着和接触性皮炎等多种皮肤病，但其潜在机制仍不清楚。此外，包括二嗪农（DZN）在内的有机磷农药（OPs）会影响 ATPase 等细胞通路，导致线粒体能量不足，甚至导致细胞功能凋亡。细胞暴露于 OP 杀虫剂 DZN 处理后，我们评估了基因表达和 DNA 损伤的变化。我们利用 AutoDock、蛋白质数据库、STRING、Way2Drug 和比较毒物基因组学数据库和工具进行了生物信息学分析。本研究采用了 MTT 试验、伤口愈合、DAPI 染色、流式细胞术和实时 PCR 技术。结果显示，DZN处理组HFFF2细胞的活力和迁移能力下降，凋亡率上升。这些研究结果表明，DZN调节了DZN细胞中凋亡基因的表达。

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来源期刊

Journal of Biochemical and Molecular Toxicology 生物-毒理学

CiteScore

5.80

自引率

2.80%

发文量

277

审稿时长

6-12 weeks

期刊介绍： The Journal of Biochemical and Molecular Toxicology is an international journal that contains original research papers, rapid communications, mini-reviews, and book reviews, all focusing on the molecular mechanisms of action and detoxication of exogenous and endogenous chemicals and toxic agents. The scope includes effects on the organism at all stages of development, on organ systems, tissues, and cells as well as on enzymes, receptors, hormones, and genes. The biochemical and molecular aspects of uptake, transport, storage, excretion, lactivation and detoxication of drugs, agricultural, industrial and environmental chemicals, natural products and food additives are all subjects suitable for publication. Of particular interest are aspects of molecular biology related to biochemical toxicology. These include studies of the expression of genes related to detoxication and activation enzymes, toxicants with modes of action involving effects on nucleic acids, gene expression and protein synthesis, and the toxicity of products derived from biotechnology.