UCL23 hierarchically regulated by WRKY51-miR528 mediates cadmium uptake, tolerance, and accumulation in rice.

IF 6.9 1区生物学 Q1 CELL BIOLOGY Cell reports Pub Date : 2025-03-25 Epub Date: 2025-02-21 DOI:10.1016/j.celrep.2025.115336

Jingai Tan, Lantian Zhang, Chuanjia Liu, Zheyuan Hong, Xia Wu, Yaqi Zhang, Muhammad Fahad, Yuxin Shen, Jianmin Bian, Haohua He, Dezhi Wu, Qingyao Shu, Jinsong Bao, Liang Wu

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Abstract

In humans, cadmium (Cd) toxicity caused by contaminated environments is associated with numerous chronic diseases. Breeding rice with low Cd accumulation is now deemed critical for sustainable agriculture development. Here, we elucidate the crucial functions of UCLACYANIN 23 (UCL23), a small copper protein, in Cd absorption, tolerance, and accumulation through modulation of reactive oxygen signals in rice. Additionally, we demonstrate that WRKY51 binds to promoters of UCL23 and miR528, a post-transcriptional regulator of UCL23, thereby contributing to Cd regulation in a dual-modulatory manner. Furthermore, we show that the natural variation of UCL23 is important for the differential accumulation of Cd in rice grains. Finally, we reveal that Indica rice harboring the major Japonica haplotype of UCL23 significantly reduces Cd uptake in roots and Cd accumulation in grains. Together, our study not only reveals a regulatory cascade in Cd regulation but also provides valuable resources for breeding low-Cd rice cultivars.

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WRKY51-miR528调控的UCL23介导水稻对镉的吸收、耐受和积累。

在人类中，由污染环境引起的镉（Cd）毒性与许多慢性疾病有关。培育低镉积累的水稻现在被认为是可持续农业发展的关键。本文通过对水稻活性氧信号的调控，阐明了铜小蛋白UCLACYANIN 23 （UCL23）在镉吸收、耐受和积累中的重要功能。此外，我们证明WRKY51结合UCL23的启动子和miR528 （UCL23的转录后调节因子），从而以双调节方式促进Cd调节。此外，我们发现UCL23的自然变异对水稻籽粒Cd的差异积累具有重要意义。最后，我们发现，携带UCL23主要单倍型的籼稻显著降低了根系对Cd的吸收和籽粒中Cd的积累。本研究不仅揭示了Cd调控的级联机制，而且为低Cd水稻品种的选育提供了宝贵的资源。

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.